Methylenetetrahydrofolate reductase (MTHFR-677 and MTHFR-1298) genotypes and haplotypes and plasma homocysteine levels in patients with occlusive artery disease and deep venous thrombosis.
Journal Title: Acta Biochimica Polonica - Year 2008, Vol 55, Issue 3
Abstract
The aim was to investigate different genotypes and haplotypes of methylenetetrahydrofolate reductase (MTHFR-677, -1298) and plasma concentration of total homocysteine (tHcy) in Macedonian patients with occlusive artery disease (OAD) and deep venous thrombosis (DVT). Investigated groups consists of 80 healthy, 74 patients with OAD, and 63 patients with DVT. Plasma tHcy was measured with Microplate Enzyme Immunoassay. Identification of MTHFR genotypes and haplotypes was done with CVD StripAssay. The probability level (P-value) was evaluated by the Student's t-test. Plasma concentration of tHcy in CC and CT genotypes of MTHFR C677T was significantly increased in patients with OAD and in patients with DVT. Plasma concentration of tHcy in AC genotype of MTHFR A1298C was increased in patients with OAD and in patients with DVT. Plasma concentration of tHcy was significantly increased in AA genotype of patients with OAD, but not in patients with DVT. We found a significant increase of plasma tHcy in patients with OAD in comparison with healthy respondents for normal:heterozygote (CC:AC), heterozygote:normal (CT:AA), and heterozygote:heterozygote (CT:AC) haplotypes. Plasma concentration of tHcy in patients with DVT in comparison with healthy respondents was significantly increased for normal:normal (CC:AA), normal heterozygote (CC:AC), and heterozygote:heterozygote (CT:AC) haplotypes. We conclude that MTHFR C677T and MTHFR A1289C genotypes and haplotypes are connected with tHcy plasma levels in Macedonian patients with OAD and DVT.
Authors and Affiliations
Igor Spiroski, Sashko Kedev, Slobodan Antov, Todor Arsov, Marija Krstevska, Sloboda Dzhekova-Stojkova, Gordana Bosilkova, Stojanka Kostovska, Dejan Trajkov, Aleksandar Petlichkovski, Ana Strezova, Olivija Efinska-Mladenovska, Mirko Spiroski
Keywords
Related Articles
- EP ID EP84205
- DOI -
- Views 86
- Downloads 0
Methylenetetrahydrofolate reductase (MTHFR-677 and MTHFR-1298) genotypes and haplotypes and plasma homocysteine levels in patients with occlusive artery disease and deep venous thrombosis.
The aim was to investigate different genotypes and haplotypes of methylenetetrahydrofolate reductase (MTHFR-677, -1298) and plasma concentration of total homocysteine (tHcy) in Macedonian patients with occlusive artery d...
Spectrophotometric assay of renal ouabain-resistant Na(+)-ATPase and its regulationby leptin and dietary-induced obesity.
Apart from Na(+),K(+)-ATPase, a second sodium pump, Na(+)-stimulated, K(+)-independentATPase (Na(+)-ATPase) is expressed in proximal convoluted tubule of the mammalian kidney. The aim ofthis study was to develop a method...
Sulforaphane-mediated induction of a phase 2 detoxifying enzyme NAD(P)H:quinonereductase and apoptosis in human lymphoblastoid cells.
The effect of sulforaphane on human lymphoblastoid cells originating froma patient of a high cancer risk was studied. Sulforaphane (SFN) is a naturally occurring substance ofchemopreventive activity. In our study, change...
The role of alkyl chain length in the inhibitory effect n-alkyl xanthates on mushroom tyrosinase activities.
Sodium salts of four n-alkyl xanthate compounds, C(2)H(5)OCS(2)Na (I), C(3)H(7)OCS(2)Na (II), C(4)H(9)OCS(2)Na (III), and C(6)H(1)3OCS(2)Na (IV) were synthesized and examined for inhibition of both cresolase and catechol...
Nitric oxide -- superoxide cooperation in the regulation of renal Na(+),K(+)-ATPase.
The aim of this study was to investigate whether endogenous superoxideanion is involved in the regulation of renal Na(+),K(+)-ATPase and ouabain-sensitive H(+),K(+)-ATPaseactivities. The study was performed in male Wista...