Sulforaphane-mediated induction of a phase 2 detoxifying enzyme NAD(P)H:quinonereductase and apoptosis in human lymphoblastoid cells.
Journal Title: Acta Biochimica Polonica - Year 2004, Vol 51, Issue 3
Abstract
The effect of sulforaphane on human lymphoblastoid cells originating froma patient of a high cancer risk was studied. Sulforaphane (SFN) is a naturally occurring substance ofchemopreventive activity. In our study, changes in cell growth, induction of apoptosis and phase 2 enzymesas well as glutathione level were examined. Apoptosis was tested by confocal microscopy at three stages:change in mitochondrial membrane potential, caspase activation and phosphatidylserine externalization.We show that SFN increases the activity of the detoxification system: it increases quinone reductaseactivity at low concentration (0.5-1 microM) and raises glutathione level in a dose-dependent manner.At higher doses (2.5-10 microM) sulforaphane is a cell growth modulator, as it caused cell growth cessation(IC50 = 3.875 microM), and apoptosis inducer. The results obtained suggest that sulforaphane acts asa chemopreventive agent in human lymphoblastoid cells.
Authors and Affiliations
Teresa Kasprzycka-Guttman, Jan Lubiński, Katarzyna Skupińska, Elzbieta Kowalska, Irena Misiewicz
Keywords
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