Microbial production and osmac study of Streptomyces species coded as G-16
Journal Title: Journal of Pharmaceutical Sciences and Research - Year 2009, Vol 1, Issue 2
Abstract
The previously isolated Streptomyces species coded as G16 was used in the present study. The test media selected were starch casein broth (SCB), GMYP broth and Soya been meal broth (SOB). The seed inoculums were transferred in conical flask were kept on rotary shaker at room temperature (175 rpm) for 5 days. The test was carried out in triplicate. The antibiotic was produced by shake flask fermentation. Starch casein and GMYP broth showed more production at the end of 2 days of fermentation. Compared to starch casein and GMYP broth the production of antibiotic was more using Soya been meal broth. The zone of inhibition was found in all medium by cup plate method, the zone of inhibition were remained same even at the end five days of fermentation in soya bean meal broth. The culture filtrate was extracted with ethyl acetate and tested for activity by bioautography. However, the bioautography showed zone of inhibition equivalent to Rf of the extract from SCB as standard and the spots were close to each other. In addition, the TLC profile of the extract collected from GMYP and SOB medium shows three spots when compared with SCB. From the results, it has been concluded that the OSMAC approach definitely changed the number of components produced in one media to the other. Any way, further confirmation has to be done by individually collecting each component and analyze their chemical structure. The study can be continued in this aspect near future, which may be beyond the scope at present.
Authors and Affiliations
Saleem TSM, Ravi V, Gauthaman K, Saisivam S
Keywords
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