Comparative study on the suitability of different substrates for Tannin Acyl Hydrolase production using Aspergillus oryzae
Journal Title: Journal of Pharmaceutical Sciences and Research - Year 2009, Vol 1, Issue 4
Abstract
An extracellular tannase produced by the fungus Aspergillus oryzae in solid state fermentation using various agricultural by-products. Among different substrates used the maximum activity of 14.40 U/g/min was found inrice bran. Other substrates produced in low level. The activity was increased when purified by ammonium sulphate (60-80%), dialysis and DEAE Sephadex A-50 column chromatography and the yield was (62.57 U/g/min). The optimal conditions for culture fermentation were 30⁰ C for 96 hrs at pH 5.5 Carbon and nitrogen sources were found to enhance tannase production when added as culture supplementations.
Authors and Affiliations
Paranthaman R, Vidhyalakshmi R, Singaravadivel K
Keywords
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