microRNA in Patients with Hepatitis B and Hepatitis C Virus Associated Hepatocellular Carcinoma and Cirrhosis
Journal Title: Viral Hepatitis Journal - Year 2019, Vol 25, Issue 3
Abstract
Objectives: The aim of this study was to determine the potentials of hsa-microRNAs (miRNA)-21-3p, hsa-miRNA-29a-3p, hsa-miRNA-122-3p, hsa-miRNA-192-5p in Hepatitis B (HBV) and HCV related hepatocellular carcinoma (HCC) and liver cirrhosis (LC) cases, being biomarkers by examining their levels. Materials and Methods: Sixty patients and 26 healthy volunteers were included in the study. Total RNA isolation in the serum samples was performed with the Direct-zolTM RNA MiniPrep (Zymo Research Corp., USA) commercial kit followed by cDNA synthesis and real-time polymerase chain reaction amplification with EPIK miRNA Select Hi/Lo-ROX (Bioline Reagents Ltd., USA). For amplification and analysis, Rotor-Gene Q (QIAGEN, Germany) instrument was used and statistical analyzes were performed with SPSS 21 (IBM, USA) program. Results: Hsa-miRNA-21-3p and hsa-miRNA-122-3p levels increased 3-4 fold in patients without HCV related LC (HCV-LC) and hsa-miRNA- 29a-3p expression in HCV infected patients has significantly decreased (p<0.05). hsa-miRNA-192-5p showed a 3-fold increase in HBV related LC (HBV-LC) group (p<0.05) but not in other groups. The hsa-miRNA-122-3p value is increased in HCV-LC patients. Conclusion: In our study, miRNA-21-3p and hsa-miRNA-122 for HBV-HCC and HCV-HCC diseases; hsa-miRNA-21-3p, hsa-miRNA- 122 and hsa-miRNA-192-5p for HBV-LC; miRNA-29a-3p test for HCV-LC could be used as diagnostic markers.
Authors and Affiliations
Gökhan Küçükkara, Ferhat Gürkan Aslan, Bilal Toka, Mehmet Köroğlu, Mustafa Altındiş
Keywords
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- EP ID EP676550
- DOI 10.4274/vhd.galenos.2019.2019.0027
- Views 169
- Downloads 0
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