Abstract

Establishment of an effective and reproducible protocol is one of the basic prerequisites for the improvement of any crop. Double-Haploid technology has proven to be breakthrough in many crop species and results in the production of homozygous plants in a single step. In spite of a number of success reports of plant tissue culture, technology, very few satisfying and reproducible protocols were available in legumes like a lentil. This study, carried out forthe production of DH plants in the different medium, with various hormone treatments in order to establish a reproducible protocol for microspore regeneration of the cultivar HUL-57. For this determination, the effects of 2 different mediums with 2 hormones on microspore of lentil either through callus induction or directly regeneration were studied. The experiment was done with LS and N6 medium in presence of BAP and 2,4-D results in the non-significant induction of callus from microspores. From the current experiment, this is found that either of the media in various hormones (BAP and 2,4-D) concentrations are non-responsive and became difficult even after combination of different stresses like cold and centrifugation. These reports of non-responsive behavior will be pointing towards trying different medium with the same objective to get the completely homozygous plants

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