Minimum Biofilm Eradication Concentration (MBEC) Assay of Silver and Selenium Nanoparticles against Biofilm forming Staphylococcus aureus
Journal Title: Journal of Medical Science And clinical Research - Year 2017, Vol 5, Issue 4
Abstract
Staphylococcus aureus is a most important bacterium that causes nosocomial infections and the etiologic agent of a wide range of diseases related with major mortality and morbidity. Total 36 positive clinical samples viz. urine, blood and pus collected from different patients were found to harbor Staphylococcus aureus with a maximum isolation from pus samples i.e. 30 (83.33%) and minimum isolation from urine samples i.e. 2 (5.56%). The degree of capacity to biofilm forming Staphylococcus aureus isolates to different phenotypic analysis of biofilm formation by Congo red agar (CRA) Nil, Tube method (TM) 5 (13.89%), and Tissue culture plate method (TCP) 12 (33.33%). The application of silver and Selenium nanoparticles as antimicrobials are gaining relevance in the medical field. Silver nanoparticles, due to their unique properties, use in day-by-day many applications in human life. The major uses of silver nanoparticles in the clinical and medical fields consist of investigative applications and curative applications. Selenium metal is an essential micronutrient for human beings and animals. Selenium nanoparticles showed the highest bactericidal and antimicrobial properties. Minimal biofilm eradication concentrations (MBEC) were determined by 96-well microtitre plate. The antibacterial effects of silver and selenium nanoparticles were evaluated with respect to growth, biofilm formation of Staphylococcus aureus strains. Among the three biofilm forming Staphylococcus aureus strains showed OD450 i.e. 0.019, 0.039, 0.075 value ≤0.080 for AgNPs whereas SeNPs couldn’t showed any ≤ 0.080 value against biofilm forming S. aureus strains. In case of MBEC test, AgNPs showed more affective against biofilm forming Staphylococcus aureus strains compared of SeNPs. Finally we suggested that AgNPs showing best antimicrobial activity against SeNPs.
Authors and Affiliations
Poonam Verma
Keywords
Related Articles
- EP ID EP284385
- DOI -
- Views 76
- Downloads 0
Awareness about PCOS and the Likelihood of Its Symptoms in Adolescent Girls in a Semi-Urban Set-Up: A Cross Sectional Study
Background: Polycystic ovary syndrome is a very common gynaecological endocrinopathy which is associated with problems like menstrual irregularities, hirsutism, obesity, insulin resistance, acne, and in later life it is...
Levels of Upper Limit of Oxidative Stress Markers During Normal Pregnancy And Pre-Eclampsia
Background: The reactive oxygen species (ROS) are involved in multiple reproductive processes – maturation of oocyte, fertilization, development of embryo, pregnancy and parturition. However, the optimal levels and inter...
To study the hip circumference to neck circumference ratio and neck circumference as a risk factor for coronary artery disease
nd beyond visceral abdominal fat. Anatomically, upper-body subcutaneous fat is a unique fat depot located in a separate compartment compared with visceral adipose tissue. Aims and Objective: to determine the reliability...
Statistical Analysis of Ridge Densities in Fingerprint as a Parameter for Identification of Sex
Introduction: Study of fingerprint is known as Dactylography. It is the process of taking impression of papillary ridges of the fingertips. Objective: The present study is based on Dactylography in which ridge densities...
To Study the Efficacy on Fertility Outcome by short term Letrozole versus Extended Letrozole Regimen in Clomiphene Citrate Resistant PCOS Women
Introduction: Polycystic ovary syndrome (PCOS) is the most common condition associated with chronic anovulation affecting 4– 6% reproductive age group, letrozole has been used successfully for ovulation induction in pati...