Mitochondrial Diversity and Time Divergence of Commonly Cultured Cichlids in Nigeria
Journal Title: Biotechnology Journal International - Year 2016, Vol 13, Issue 2
Abstract
Aim: The aims are to study the genetic diversity and population structure of Tilapia fish species in Nigeria using mtDNA D-loop region and time divergence of these various groups of fish to give a baseline information for fish management and conservation programs. Methodology: Blood samples from four species of tilapia were collected with the use of FTA cards for DNA extraction and PCR amplification. A 941bp long fragment, including the D-loop, was subsequently used for analysis. Molecular diversity indices and Tajima’s selective test were determined. A phylogenetic tree was constructed for all the four fish species using UPGMA. The divergence time among the four fish species using MEGA5 software. Place and Duration of Study: The study was carried out in the Biotechnology Centre, Federal University of Agriculture, Abeokuta Nigeria between. Genotyping and Optimization of PCR conditions were carried out at STAB VIDA Lda, Madan Parque, Rua dos Inventores, 2825-182 Caparica Portugal. Total duration of study was between February to August 2015. Results: Sarotherodon melanotheron has the highest nucleotide diversity (π), mean number of pairwise differences and number of polymorphic site, while the least was observed in Sarotherodon galiIaeus. The highest number of monomorphic site and the highest sequences conservation percentage was observed in Sarotherodon galiIaeus. The Neighbour joining tree revealed six (6) clusters with no clear sub-structuring among the sampled fish populations. The smallest divergence time (about 31 mya) was observed between Sarotherodon galilaeus and Sarotherodon melanotheron. Sarotherodon spp diverged from Oreochromis niloticus about 32.5 mya. Conclusion: The present study revealed that the sampled Tilapia species from Nigeria represent a single stock. The existing low levels of genetic variability observed here may compromise the evolutionary plasticity of the Tilapia species population. These findings indicate an urgent need for the careful monitoring of the harvesting of Tilapia species in Nigeria.
Authors and Affiliations
O. T. Agbebi, C. J. Echefu, I. O. Adeosun, A. H. Ajibade, E. A. Adegbite, M. B. Ilori, S. O. Durosaro
Keywords
Related Articles
- EP ID EP237884
- DOI 10.9734/BBJ/2016/25470
- Views 128
- Downloads 0
Purification and Characterization of α-Glucan Phosphorylase Isoform Pho 2 from Spinach Leaves
α-Glucan phosphorylase is an important enzyme of carbohydrate metabolism. In spinach leaves, it has been reported in two multiple forms viz. Pho 2 (cytosolic) and Pho 1 (plastidial). Here, we extracted and purified Pho 2...
Identification of Bacterial Population of Activated Sludge Process and Their Potentials in Pharmaceutical Effluent Treatment
Aims/objectives: The cognition about microbial population of activated sludge and their treatment potential will be very useful for industrial wastewater treatment plant operation. Methodology: In this study microbial p...
Alteration in Trace Elements Concentration and Possible Copper Toxicity in Juiced Leafy Vegetables Commonly Consumed in Southwestern Nigeria
This study investigated the effects of juicing on four micro-minerals (Cu, Zn, Mn and Mo) contents of six different leafy vegetables commonly consumed in South western, Nigeria. Micro-minerals were determined using Atomi...
DNA Sequence Characteristics and Phylogenetics of Three Oligonucleotides Markers on Clariid Species
Aims: The aim of this study is to express the profiles of three oligonucleotide markers corresponded to reproductive genes that may be different between the two Clariid species (Clarias gariepinus and Heterobranchus bido...
Genetic Variations in Asparagus racemosus, an Endangered Medicinal Herb Endemic to India Using RAPD Markers
Aim: To study the genetic diversity in Asparagus racemosus germplasm using RAPD molecular markers for its better conservation and utilization. Study Design: RAPD markers used to check genetic diversity in Asparagus racem...