Modeling the process of microbial biofilm formation on stainless steel with a different surface roughness
Journal Title: Восточно-Европейский журнал передовых технологий - Year 2019, Vol 2, Issue 11
Abstract
<p>This paper reports a study into the process of<strong> </strong>bacteria adhesion to surfaces of different roughness depending on sizes and shapes. It was determined that at the surface of stainless steel with a roughness of 2.687±0.014 µm, the film formation process in E. coli and S. aureus occurred equally over 3 to 24 hours and did not depend on the size of bacteria. This makes it possible to argue that rod-shaped and coccal bacteria freely attach themselves in the grooves of roughness, followed by the initial process related to the first stage of a biofilm formation. During sanitization, the hollows of roughness could host both the coccal and rod-shaped bacteria. At the surface of steel with a roughness of 0.95±0.092 μm the process of film formation in S. aureus occurred more intensely than in E. coli. Within 3 h of incubation, density of the formed biofilms S. aureus was 1.2 times larger compared to the biofilms E. coli. Over the following 15 hours of incubation, the biofilms S. aureus were on average 1.3 times denser. This suggests that S. aureus, due to a spherical form, can stay put in the hollows of roughness of 0.95±0.092 µm and quicker attach to the surface. At the same time, E. coli, owing to a rod-shaped shape, would attach at such a surface roughness to the hollows lengthwise only. It has been proven that at a surface roughness of 0.63±0.087 µm the intensity of the S. aureus film formation was on average 1.4 times faster than in E. coli. However, at a roughness of 0.16±0.018 μm the process of film formation occurred equally in E. coli and S. aureus, but the biofilms demonstrated lower density compared to those that formed at a roughness of 0.63±0.087 µm.</p>Thus, the use of equipment with a roughness of less than 0.5 µm in the dairy industry will make it possible to reduce the attachment of microorganisms to surface and to decrease contamination of dairy products
Authors and Affiliations
Mykola Kukhtyn, Khrystyna Kravcheniuk, Ludmila Beyko, Yulia Horiuk, Oleksandr Skliar, Serhii Kernychnyi
Keywords
Related Articles
- EP ID EP666348
- DOI 10.15587/1729-4061.2019.160142
- Views 71
- Downloads 0
Defining the measures to rationally manage the sustainable development of agricultural land use
<p>The issue of socio-economic development of rural areas was explored. It was substantiated that the state policy should be aimed at mobilizing labor, financial, material and organizational resources, strengthening the...
The aerated concrete based on an integrated foam concentrate containing iron compounds
<p>The chemical processes of Portland cement hydration enable the formation of concrete compressive strength. Under certain conditions, increasing the rate of cement hydration contributes to the improved strength of conc...
Application of piecewisecubic functions for constructing a Bezier type curve of C1 smoothness
<p class="a">We have proposed and implemented a new method for constructing a spline curve of third degree, which possesses the properties of both a cubic spline and the Bezier curve. Similar to building the Bezier curve...
Cryoscopic and microbiological study of the semifinished product for making a smoothie drink
<p class="a">The dynamics of freezing a semi-finished product for making a smoothie drink was investigated. We have chosen, as the subject of present study, a semi-finished product whose production technology included st...
Determination of optimal parameters of the pulse width modulation of the 4qs transducer for electriс rolling stock
<p>Operating modes of the single-phase 4qs transducer with pulse-width modulation as part of the AC electric rolling stock are investigated. The method is developed for determining the PWM parameters, at which the optimu...