Modification of the Uranyl Acetate Replacement Staining Protocol for Transmission Electron Microscopy
Journal Title: International Journal of Medical Nano Research - Year 2016, Vol 3, Issue 1
Abstract
A uranyl acetate replacement staining protocol has eliminated the need for highly toxic uranyl acetate in the electron microscopy laboratory. However, the method results in undesirable charging effects when viewed under the transmission electron microscope and can be further improved. A modification of the uranyl acetate replacement staining protocol that simplifies the process is described. The modified method takes less time, and produces better contrast and less charging effects compared to the existing uranyl acetate replacement staining protocol.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP352247
- DOI 10.23937/2378-3664/1410013
- Views 120
- Downloads 0
Protease Biosensors Based on Peptide-Nanocellulose Conjugates: From Molecular Design to Dressing Interface
The development of point of care diagnostic protease sensors applied to wound healing has received increased interest in chronic wound treatment as an interface for chronic wound dressings. Biosensor technology and the u...
Modification of the Uranyl Acetate Replacement Staining Protocol for Transmission Electron Microscopy
A uranyl acetate replacement staining protocol has eliminated the need for highly toxic uranyl acetate in the electron microscopy laboratory. However, the method results in undesirable charging effects when viewed under...
Silver Nanoparticles for the Rapid Healing of Diabetic Foot Ulcers
Diabetic foot ulcers are one of the major complications of patients with diabetes mellitus. And due to their high susceptibility to microbial infections, are the leading cause of hospitalization and amputation of lower l...
Estimating Binding Capability of Radiopharmaceuticals by Cell Culture Studies
Radiopharmaceuticals have applications in biologic research, drug discovery, diagnosis of human disease and molecular therapeutics for a wide variety of medical conditions. With the increasingly central role of radiotrac...
Nanoparticles: From Diagnosis to Therapy
Cancer is a leading cause of death, the second most common cause, exceeded only by heart disease. Still, the current clinical imaging methods and treatments are in many situations unable to provide timely detection and c...