Modified Germ Tube Test: A Rapid Test for Differentiation of Candida Albicans from Candida Dubliniensis
Journal Title: International Journal of Contemporary Medical Research - Year 2018, Vol 5, Issue 3
Abstract
Background: C. albicans, the most prevalent yeast isolated from clinical samples, whose presumptive identification is usually based on germ tube test using human serum at 37°C. But C. dubliniensis also produces true germ tubes in same conditions. So a simple and fast method is needed to differentiate C. albicans from C. dubliniensis. In the present study we evaluated germ tube formation on YEPD medium at 39°C for rapid differentiation of the two species. Materials and Methods: A total of 200 strains were tested for germ tube formation on YEPD medium at 39°C which included 186 strains of C. albicans and 14 strains of C. dubliniensis. Standard strains of both species were included in the study. Results: Out of 186 test strains of C. albicans 166 (89.3%) formed true germ tubes after 1 h at 39°C while the remaining grew as a yeast form. All the 20 test strains of C. dubliniensis grew as a yeast form. Conclusion: Positive modified germ tube test can be used for presumptive identification of C. albicans and ruling out C. dubliniensis however negative test cannot be used to differentiate between the two species.
Authors and Affiliations
Abiroo Jan, Gulnaz Bashir, Rubhana Qadir, Bashir A Fomda, Sofia . , Aamir Yaqoob Hakak
Keywords
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