Modified Petroff’s Method: an Excellent Simplified Decontamination Technique in Comparison with Petroff’s Method
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2014, Vol 10, Issue 3
Abstract
Tuberculosis is a major public health problem. In India, about 40% of the population is infected with TB bacillus. For diagnosis of TB, smear microscopy is still the most used due to its simplicity though it lacks sensitivity as compared with culture. This study compared the original Petroff’s method with Modified Petroff’s method. Spot and early morning sputum samples of 225 suspected tuberculosis cases were collected and processed by petroff’s and Modified Petroff’s method separately. Treated samples were cultured in Lowenstein-Jensen media. Out of 225 processed sputum specimens, 123 (54.67%) were smear positive by direct, 127 (56.44%) by Petroff’s method and 129 (57.33%) by Modified Petroff’s method. The number of culture positives by Modified Petroff’s method was 55.56%, by Petroff’s method were 53.33% and 45.33% by Direct method. Culture positives by Modified Petroff’s method compared with Direct method are statistically significant (P=0.0249, Chi square test). Negative culture and contamination rate was minimum with Modified Petroff’s method. Modified Petroff’s method requires lesser time, has lower contamination rate and higher yield in culture positivity as compared with Petroff’s method, making it a more suitable and better method of concentration and decontamination.
Authors and Affiliations
Kiran Tripathi , Purti C. Tripathi , Shashwati Nema , Arun Kumar Shrivastava , Kalpana Dwiwedi , Ashok Kumar Dhanvijay
Keywords
Related Articles
- EP ID EP152205
- DOI -
- Views 112
- Downloads 0
Comparison of intra ocular pressure changes in third trimester of normal pregnancy and pregnancy induced hypertension
12-17% pregnant women face various problems related to pregnancy and child birth. The alarming symptoms of eye with Pregnancy Induced Hypertension are blurring, dimness of vision and at times complete blindness. 60 pregn...
Protein carbonylation, Lipid peroxidation and serum alpha Tocopherol activity in Preeclampsia
Hypertensive disorders are the most common medical complication of pregnancy and are important cause of maternal and perinatal morbidity and mortality. The etiology of preeclampsia has always remained elusive. More recen...
A study of clinical profile and complications of Poisonous snake bites in a tertiary care hospital in south India
Background: Snake bite is still a major public health hazard which is preventable and treatable. The clinical features of snake bite envenomation are highly varied. Aims: To study the clinical profile and complications o...
Cutaneous Larva Migrans: Diagnosis on Fine Needle Aspiration
Cutaneous larva migrans is a skin disease in humans caused by larva of nematode parasites of hookworm family. The diagnosis of Cutaneous larva migrans is based mainly on clinicalfeatures and history. On review of litera...
Anticoagulant Induced Pseudothrombocytopenia- An Incidental Finding
Thrombocytopenia may be caused either by impaired platelet production or due to accelerated platelet destruction. In some cases, there is an erroneously low reading of platelet counts in the automated analyzers, without...