Modulation of antiviral cytokine production and lung protection against influenza virus by the glycosides from Ligustrum purpurascens
Journal Title: Toxicology Communications - Year 2021, Vol 3, Issue 1
Abstract
Background: Ligustrum purpurascens has been used as a traditional herb for over 2,000 years in China. This study was design to investigate the modulation of antiviral cytokines and reduction in lung inflammation of virus-infected mice by the glycosides isolated from Ligustrum purpurascens. Methods: Ligustrum purpurascens glycosides (LPG) were isolated from the leaves of Ligustrum purpurascens. Proliferation of spleen lymphocytes were investigated after LPG treatment. The in vitro and in vivo cytokine modulation of LPG was studied. Furthermore, the anti-inflammatory and antiviral activities of LPG, with the potential to reduce inflammatory lung disorders, were investigated by influenza A virus infected mice. Results: LPG could significantly promote the proliferation, and also could stimulate the production of IFN-γ by spleen lymphocytes in a dose-dependent manner. IFN-γ expression level was increased significantly compared to the control and presented a dose-dependent manner in vitro. Furthermore, LPG inhibit the expression of TNF-α and IL-10, which return to normal level in the cyclophosphamide -induced mice model in vivo. Besides, the histopathological analysis indicated LPG reduced acute lung injury in mice infected with influenza virus. Conclusion: This study suggested that LPG could increase the exression of IFN-γ, immunoregulation and decrease lung inflammation of virus-infected mice.
Authors and Affiliations
Chen-Yang Li, Xiao-Jun Zhong, Xiao-Peng Hu, Qin-Chang Zhu, Hai-Tao Xiao, Peng Zhai, Da-Hong Yao, Muhammad Shahid Riaz, Zhen-Dan He, Xun Song
Keywords
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- EP ID EP692821
- DOI 10.12032/ATR20210203
- Views 134
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