MOFs-derived Fe-N-C nanozyme for colorimetric detection of hdroquinone

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MOFs-derived Fe-N-C nanozyme for colorimetric detection of hdroquinone

Journal Title: China Powder Science and Technology - Year 2024, Vol 30, Issue 4

Abstract

Objective Hydroquinone is a phenolic compound widely used in industry. It is difficult to degrade in the aquatic ecological environment and is harmful to human health. Therefore， constructing a simple and sensitive method for the detection of hydroquinone is of great significant. Methods In this study， an MOFs-derived Fe-N-C catalyst was synthesized through a simple chemical doping method and high-temperature pyrolysis， using an Fe-ZIF-8 precursor. The physicochemical properties of Fe-N-C were characterized in detail through SEM， TEM， XRD， FTIR， and XPS. The effect of the introducing Fe3+ on the enzyme activity of the catalyst was studied. The enzyme-like activity， catalytic mechanism， and kinetic parameters of Fe-N-C were systematically investigated. Based on the enzyme-like activity of Fe-N-C， a colorimetric sensor for the detection of hydroquinone was developed. Results and Discussion Based on the aforementioned characterization and experimental findings， Fe-N-C exhibited excellent peroxidase-like activity and weak oxidase-like activity. In addition， in the presence of hydrogen peroxide， OPD and ABTS as substrates were also oxidized to yellow and blue products by Fe-N-C， with characteristic absorption peaks at 448 nm and 416 nm， respectively. Additionally， the poisoning experiment with KSCN showed that Fe-Nx was the main active site in Fe-N-C catalyst. The study of the catalytic mechanism confirmed that ·OH， O2•- and 1O2 were active oxygen radicals playing a major role in the catalytic oxidation of TMB. The catalytic activity of Fe-N-C nanozymes was further studied through steady-state kinetic analysis. The Km and Vmax of Fe-N-C for TMB were 0.134 mmol/L and 0.754 × 10-7 M·s-1， respectively， while those for H2O2 were 16.535 mmol/L and 2.533 × 10-7 M·s-1， respectively. Finally， the colorimetric sensor detected HQ in a linear range of 0~33 μmol/L with a detection limit of 0.356 μmol/L. Through anti-interference experiments， the established colorimetric sensing platform showed robust anti-interference ability and selectivity in detecting hydroquinone. Conclusion The introduction of Fe3+ significantly improves the enzyme-like activity of N-C nanomaterials. Fe-N-C exhibits excellent peroxidase-like activity， which can rapidly oxidize the chromogenic substrate 3，3'，5，5'-tetramethylbenzidine （TMB） to blue. Fe-Nx is the main active site of Fe-N-C nanozymes， and hydroxyl radical（•OH）， superoxide radicals （O2•-） and singlet oxygen （1O2） are the main reactive oxygen species （ROS）. Hydroquinone is a strong reducing organic pollutant that can reduce blue oxTMB to a colorless state. Based on this， a sensing platform for colorimetric detection of HQ was constructed. This method has good sensitivity and selectivity for hydroquinone， which expands the application of MOFs-based nanozymes in the field of environmental pollutant detection.

Authors and Affiliations

Yuanjie Zhang, Jinkai Li, Zongming Liu

Keywords

EP ID EP749277
DOI 10.13732/j.issn.1008-5548.2024.04.012
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