Molecular and Serological Studies for Detection and Identification of Cotton Leaf Curl Virus in Cotton Plant (G. barbadense L.) in Egypt
Journal Title: Microbiology Research Journal International - Year 2015, Vol 9, Issue 3
Abstract
Aims: Cotton production contributes in economic security in Egypt. Cotton leaf curl virus (CLCuV) belongs to the Begomovirus genus and Geminiviridae family that is transmitted by the whitefly Bemisia tabaci. It is the greatest current threat where cotton is grown. In Egypt, a distinct Begomovirus causing leaf curl has been identified in okra, tomato, hollyhock and other malvaceous species suggesting that it could possibly infect Egyptian cotton. The aim of this study was the detection and isolation of this Virus from Egyptian cotton fields. Methodology: Diseased leaves of cotton, okra and tomato plants were collected from different localities during 2009; samples were subjected to DAS-ELISA for viral detection. Coat protein (CP) gene of the virus was amplified by PCR, sequenced and aligned with the others Begomovirus available in the GenBank. Cloning and sub cloning of CP gene for cotton virus were performed and its recombinant protein was separated on SDS-PAGE. DBIA was also used to identify protein of the virus. Results: The amplified fragment of the CP gene was about 280bp in all samples. The CP gene of CLCUV in our study shared 97% identity with CLCuV isolate from Pakistan while, CP gene amplicon of okra leaf curl virus (OLCUV) showed identity of 84% with three isolates of tomato leaf curl virus (TYLCV) from Chian. The recombinant protein of CLCUV was about 24kDa and positive relatedness was shown between this recombinant protein and TYLCuV antiserum. Conclusion: our study showed the existence of such virus in malvaceous species as okra plant may be causes a serious problem for cotton production in Egypt. Also, the CP gene is a good tool for examination the existence of the virus in the infected fields. The purified protein of CLCUV can use as an antigen to produce its antibodies.
Authors and Affiliations
Azza Makhlouf, D. G. Asse, E. E. Hafez, M. A. EL Seehy
Keywords
Related Articles
- EP ID EP354376
- DOI 10.9734/BMRJ/2015/18939
- Views 88
- Downloads 0
Impact of Endomycorrhizae and Acidithiobacillus ferrooxidans with Sulfur and Phosphorus Nutrition on Onion (Allium cepa L.) and Maize (Zea mays L.) Plants under Field Conditions
Aim: To evaluate the effects of endomycorrhizal fungi (AMF) and sulfur oxidizing bacteria as biofertilizers in presence of phosphors and sulfur as mineral nutrients on onion (Allium cepa L.) and maize (Zea mays L.) plant...
Microbiological and Physico-chemical Analyses of Borehole Water Samples from Private Schools in Umuahia Metropolis, Abia State, Nigeria
The microbiological and physico-chemical analysis of randomly selected borehole waters used by staffs, students and pupils in twenty (20) private schools in Umuahia Abia State were carried out. Five bacteria: Escherichia...
Antibacterial and Antioxidant Potential of Leaf and Seed Extracts of Murraya koenigii (Linn.) Spreng
India is known for its rich biodiversity and traditional healers have used flora to cure various human ailments. The same plants are still being used today for the benefit of mankind. Since the beginning of this century,...
Immobilization and Thermostability of Manganese Peroxidase from Trichoderma harzianum
The aim of the present investigation was to isolate Manganese peroxidase (MnP) (EC 1.11.1.13) from Trichoderma harzianum. The optimal pH and temperature for the enzyme production were 5.0 and 30°C. The best...
Microbiological Quality of Cows' Milk Butter Processed in Khartoum State, Sudan
Aims: This investigation was carried out to study the microbiological quality of butter produced in Khartoum State, Sudan. Methodology: Butter was manufactured traditionally by farmers in Khartoum north (T1) and Omdurma...