Molecular biological analysis of RhD variant blood donors in Zhengzhou
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 8
Abstract
Objective To investigate the serological characteristics and gene mutation mechanism of RhD variant blood donors in Zhengzhou. Methods From January 2023 to December 2023, 1 619 RhD-negative blood donors sent to our laboratory were selected for the study, and RhD negative confirmation test and RhCE phenotype detection were applied by tube method and microcolumn gel indirect antiglobulin test method. RHD gene amplification and Sanger sequencing were used to detect RhD variant sample genotypes. Results A total of 69 cases of RhD variants were detected in the RhD negative confirmation test, with a proportion of 4.26%(69/1 619).The RhCE phenotypes were ccEe, Ccee, CcEe and CCee.There were 17 genotypes and 15 phenotypes of the D variant.The RHD*weak partial 15 allele was the most frequent(33 cases), with a frequency of 47.83%(33/69), and the main phenotype was the ccEe. This was followed by the RHD*DVI.3 allele in 20 cases with a frequency of 28.99%(20/69) and the predominant phenotype was Ccee. The RHD*weak partial 15/RHD*01EL.01 heterozygote was found in 3 cases with a frequency of 4.35%(3/69), all with the CcEe phenotype. Other rare genotypes were present in 13 cases with a frequency of 18.84%(13/69). Antibody screening was positive in 3 cases with a frequency of 4.35%(3/69). Two cases of female blood donors, both with history of pregnancy and childbirth, were identified as anti-D; one case of male donor was anti-M. Conclusion The RHD*weak partial 15 genotype was the most common among the RhD variants in blood donors in Zhengzhou, followed by the RHD*DVI.3 genotype. It plays an important role in guaranteeing the safety of blood supply and guiding precision transfusion.
Authors and Affiliations
Hecai YANG, Qunjuan ZENG, Xiaoli MA, Yonglei LYU, Minglu GENG, Liping WANG
Keywords
Related Articles
- EP ID EP744446
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.08.004
- Views 12
- Downloads 0
Suspected hemolysis caused by type A therapeutic plasma exchange in a patient with AB subtype miss detected
Objective To investigate the serological changes of blood type and whether hemolytic reactions occurred in a patient with ABw subtype after type A therapeutic plasma exchange(TPE) retrospectively. Methods The ABO blood g...
Effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1
Objective To determine the effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1(ACKR1). Methods Samples from six bags of red blood cells product,...
Research progress of platelets and their derivatives based drug delivery systems
Platelets play an important role in physiological and pathological activities such as thrombosis, inflammation and tumorigenesis. At present, the application of platelets in drug delivery systems is increasingly studied....
Whole blood donation related adverse reactions and predisposing factors: a study of blood donors interviewed within 2 weeks after donation
Objective To study the adverse reactions(ADRs) in blood donors after whole-blood donation in terms of frequency and types, so as to explore the predisposing factors of adverse reactions. Methods A questionnaire was desig...
Plasmin-α2-plasmin inhibitor complex and thrombin-antithrombin complex in risk stratification of massive transfusion in patients with postpartum hemorrhage
Objective To analyze the value of plasmin-α2-plasmin inhibitor complex (PIC) and thrombin-antithrombin complex (TAT) for risk stratification of massive transfusion (MT) in patients with postpartum hemorrhage (PPH). Metho...