MOLECULAR CHARACTERIZATION OF LASB GENE OF PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL AND ENVIRONMENTAL SOURCES IN THI-QAR PROVINCE
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 9
Abstract
The study was conducted through a period from March to September 2016.A total of 365 samples from hospital environment and various clinical cases of different patients were randomly collected and examined for detection of Pseudomonas aeruginosa. These clinical samples included wound swabs, burn swabs, ear swabs, urine and sputum samples. All collected samples were screened for presence of Pseudomonas aeruginosa by culturing on appropriate media and 76 isolates of Pseudomonas aeruginosa were identified via biochemical tests and confirmed by API 20NE system. In attempting to identification of P.aeruginosa strains at the DNA level, Polymerase chain reaction (PCR) was used based on specific primer for 16SrRNA. The results showed that PCR has found to be rapid and sensitive and specific for identification of P. aeruginosa. In addition, 16S rRNA was used as confirmation gene, while lasB used as virulence gene.
Authors and Affiliations
Mushtaq Talib Al-Safi
Keywords
Related Articles
- EP ID EP629044
- DOI -
- Views 138
- Downloads 0
EVALUATION OF ORAL ADMINISTRATION OF NICOTINE ON LIBIDO AND SPERM QUALITIES IN MALE WISTAR RATS.
The burden of implication of Nicotine-laden cigarette on human reproductive cells and sexual performance is legendary and whether or not nicotine is directly involved remains to be unraveled hence, this study was designe...
ESTIMIATION OF SERUM HEPCIDIN LEVEL IN SUDANESE PATIENTS WITHS SICKLE CELL ANEMIA
Sickle cell disease (SCD) is general term for abnormalities of hemoglobin structurein which sickle gene is inherited from at least one parent. This genetic disorders are characterized by the production of Hb S. Acute and...
DATA INTEGRITY: AN OBLIGATORY TOOL TO SURVIVE PHARMA INDUSTRY
Medicines and health products are important for addressing health problems and improve quality of lives. They form an indispensable component of health systems in the prevention, diagnosis and treatment of disease and in...
NEW DELHI METALLO-Β-LACTAMASE 1 (NDM-1) PRODUCING ESCHERICHIA COLI IN BASRAH HOSPITALS. IRAQ
Resistance to carbapenems is developing around the world Escherichia coli(E.coli) resistance to carbapenems is associated with production of carbapenem-hydrolyzing class B metallo-β-lactamases (MBL).New Delhi metallo-β-l...
EVALUATION OF CYTOTOXIC ACTIVITY OF CHORDA FILUM FROM THE LEBANESE COAST AGAINST MCF-7 CANCER CELL LINE
The discovery of cancer drugs and the identification of agents that effectively destroy cancer cells or stop their growth without toxicity to normal cells, and reduce the opportunity for the development of drug resistanc...