Molecular Characterization of Medicinal Plant – Cassia Fistula L by Matk Gene
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2017, Vol 5, Issue 6
Abstract
The studies on taxonomy, ethnobotany and antimicrobial potential are imperative for plants with traditional medicinal value. Two important reasons for plant barcoding is to identity unknown specimens to known species and to share it with the community resource of sequences to establish its taxonomical clarification. MaturaseK gene (matK) of chloroplast is highly conserved in plants which is associated in Group II intron RNA splicing. This reputed gene product matK is the only maturase present in chloroplasts making its presence unique. DNA barcoding by plastid coding matK markers involves sequencing of a standard region of DNA as a tool for angiosperm identification. In this study, leaf samples of Cassia fistula L were collected from Chennai and Polymerase chain reaction (PCR) was performed to amplify the matK gene and molecular characterization was performed.
Authors and Affiliations
Hari Prasath B, Chandra Mohan A, Divya S. R
Keywords
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