Molecular characterization of Staphylococcus aureus - human pathogen from clinical samples by RAPD markers
Journal Title: Journal of Medical Science And clinical Research - Year 2013, Vol 1, Issue 2
Abstract
Staphylococcus aureus is one of the most significant pathogens causing nosocomial and skin infection. It is an opportunistic bacterium, frequently part of the human micro flora, causing disease when the immune system becomes compromised. Many genotypic variants and antibiotic strains have also been reported in the earlier studies. Therefore, the present study was carried out to examine the genotypic variations of S. aureus in different clinical samples. Six different samples of S. aureus isolated from Urinary tract infection (UTI), knee wound and sputum were subjected for various biochemical tests to confirm the identity of S. aureus as a pathogen. Molecular characterization of these isolates was carried out by using random amplified polymorphic DNA (RAPD). Out of five primers (D-18, D-20, T-7, W-2 and X-6) tested D-20 produced maximum number of bands. From the result obtained, it was seen that clinical isolates of S. aureus from knee wound (SA-1) and sputum sample (SA-6) are closely related. Close relation was also observed between sputum sample (SA-4) and UTI sample (SA-5). In conclusion, S. aureus strains are not specific for infection.
Authors and Affiliations
Beena Derajira Bopanna
Keywords
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