Molecular description of alpha-hemolysin producing Escherichia coli isolates from extraintestinal source
Journal Title: INNOVATIVE JOURNAL OF MEDICAL AND HEALTH SCIENCE - Year 2018, Vol 8, Issue 6
Abstract
Context:Alpha-hemolysin (Hly) is a common exotoxin produced by Escherichia coli, which plays an important role as a virulence factor in a number of clinical infections. There exist other virulence propertiesexhibited by such strains whose role in pathogenesis have not yet been clearly elucidated.Aims:Aim of presentstudy was to characterize those alpha-hemolysin producing isolates on the basis of their virulence factorsandphylogenetic background.Settings and Design:This descriptive study was carried out in a multi-specialty tertiary care hospital.Methods and Material:Three hundred non-repeat E. coli isolates from in-patients were studied. Isolates were differentiated as haemolytic and non-haemolytic on the basis of both phenotypic methodssuch as haemolysis on sheep blood agar and genotypic detection of the hly gene. Possession ofvirulence and drug resistance genes was determined by multiplex polymerasechain reaction (PCR). Phylogenetic analysis was performed by triplex PCRmethods. Antibiotic sensitivity testing was performed by disk diffusion method.Results:Of the 300 isolates, it was observed that by phenotypic (haemolysis on blood agar) method, 65(22%) were hemolytic whereas thegenotypic method detected 70 (23.5%) isolates possessing alpha haemolysin (hlyA+) marker. B2 phylogroup isolates were found to harbour the hlyA+ marker at a significantly higher(P < 0.05)rate. The possessionof papC and cnf1 genes was significantly higher in hlyA+ isolates.Conclusions:Alpha-haemolysin producing isolates demonstrated higher virulence properties. The microbiology laboratory may report haemolytic characteristicsof the isolates as it may alertthe physician of the higher pathogenic potential of the strain and to initiate appropriate treatment.
Authors and Affiliations
Dr. Arindam Chakraborty
Keywords
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