Molecular detection and antimicrobial resistance of Aeromonas from houseflies (Musca domestica) in Iran
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 3
Abstract
Objective. This study aimed to report the molecular detection and antimicrobial resistance of Aeromonas among houseflies (Musca domestica) in Shahrekord and Isfahan provinces of Iran. Materials and methods. Flies were caught from household kitchens, cattle farms, animal hospitals, human hospitals, slaughter house and poultry farms and put in collection separate sterile tubes. Isolation was accomplished by culture of flies in alkaline peptone water followed by identification with Aeromonas-specific Polymerase Chain Reaction (PCR). Results. Out of 600 houseflies 73 (12.2%) were infected with Aeromonas spp. Significantly higher frequencies of Aeromonas were isolated in Shahrekord province (13.0%; 39/300) than in Isfahan province (11.3%; 34/300). The recovery frequencies of the organisms were significantly lower in kitchens as compared to those in cattle farms and hospital wards which were similar. Higher proportions of infected flies were obtained during summer whereas low proportions were obtained during winter. Conclusions. It is concluded that houseflies do harbor diarrheagenic pathogens, including Aeromonas especially during summer. The carried organisms are resistant to a number of antimicrobials at different levels. Thus, future plans aimed at stemming infections caused by these organisms should take flies into account. Control efforts of infections caused by this particular bacterium should therefore take into account Musca domestica.
Authors and Affiliations
Davood Ommi, MD| Functional Neurosurgery Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Seyed Mohammadreza Hashemian, MD| Chronic Respiratory Disease Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran., Elahe Tajbakhsh, Ph. D| Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran., Faham Khamesipour, DVM *| Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran, P.O. Box 166.Correspondence: dr_faham@yahoo.com; f.khamesipour@iaushk.ac.ir
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