MOLECULAR DETECTION OF CTX-M-Β-LACTAMASES IN PSEUDOMONAS AEROGENOSA STRAINS ISOLATED FROM CORNEAL INFECTIONS
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 4
Abstract
Bacteria producing CTX-M- β -lactamases are emerging around the world as a source of resistance to oxyiminocephalosporins such as cefotaxime (CTX). This study was aimed to detect the presence of blaCTX-M gene among Pseudomonas aerogenosa (P. aerogenosa) strains isolated from corneal infections in Ibn- Al haitham teaching hospital for eyes in Baghdad. Eleven P. aerogenosa strains were isolated from specimens of corneal infections of patients at Ibn- Al haitham teaching hospital during four months (April- July, 2016).. The results of antibiotic susceptibility of P. aerogenosa strains showed most strains were resist to most antibiotics under test and the highest resistant antibiotic was Cefazolin which gave 11(100%) resistance rate followed by Ampicillin, Aztreonam, Ampicillin-Sulbactam and Amoxicillin-clavulanic acid which gave 9(81.81%) resistance rate. On the other hand the most effective antibiotic under test was Amikacin which it gave the lowest resistance rate 2(18.18%) followed by Chloramphinical which it gave 4(36.36%) resistance rate. Phenotypic detection of ESBL producing strains was done by screening test and the results showed that out of 11 P. aerogenosa, 7(63.64%) were positive ESBL producing strains while 4(36.36%) P. aerogenosa strains were negative ESBL producing strains. Molecular detection of blaCTX-M showed that out of 11 P. aeruginosa strains, 3(27.27%) were harbored the blaCTX gene (544bp) and those 3 strains were also positive ESBL producing strains by phenotypic detection, while 8(72.72%) strains not harbored blaCTX-M gene.
Authors and Affiliations
Dr. Buthainah Mohammed Taha
Keywords
Related Articles
- EP ID EP628225
- DOI -
- Views 178
- Downloads 0
EPISTAXIS IN CHILDREN: COMPARITIVE ANALYSIS OF TOPICAL ANTISEPTIC AND CAUTERY VERSUS TOPICAL ANTISEPTIC ALONE
Objective: To evaluate and compare different modalities in management of simple recurrent epistaxis in children. Patients and methods: A total of 148 children (age 5-14 years) presented to ENT clinic with recurrent epist...
THE RELATIONSHIP OF THE ILIOINGUINAL NERVE TO THE SPERMATIC CORD WITHIN THE INGUINAL CANAL IN SUDANESE CADAVERS
Background: Ilioinguinal nerve frequently injured during lower abdominal surgeries especially during inguinal hernia repair. Objective: To identify the variations of ilioinguinal nerve related to the spermatic cord. Mate...
ANTIOXIDANT AND FREE RADICAL SCAVENGER EFFECTS OF METHANOL LEAF EXTRACT OF LUPINUS ARBOREUS.
Objective: To investigate the antioxidant and free radical scavenger profiles of the leaf extract of Lupinus arboreus. Methods: The crude methanol extract (CME) of the dried leaves obtained by cold maceration for 72 h we...
THE INVESTIGATION OF PHYSIOLOGICAL KNOWLEDGE AND FIRST AID APPLIED TO THE CURRICULAR GRID OF PREHOSPITAL CARE IN PHYSICAL EDUCATION COURSES IN BELÉM-PA, BRAZIL, EASTERN AMAZON
The present study aims to analyze the degree of knowledge about physiology and first aid applied to the prehospital care of the graduating students of the physical education course of the main colleges of the metropolita...
LAPAROSCOPIC RESECTION OF A SIGMOID COLO - VESICAL FISTULA
Colo vesical fistula can be a result of a variety of conditions. Patients commonly present with lower urinary tract symptoms (LUTS) and recurrent urinary tract infections (UTI).The management is surgical resection. Urina...