MOLECULAR DETECTION OF CTX-M-Β-LACTAMASES IN PSEUDOMONAS AEROGENOSA STRAINS ISOLATED FROM CORNEAL INFECTIONS
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 4
Abstract
Bacteria producing CTX-M- β -lactamases are emerging around the world as a source of resistance to oxyiminocephalosporins such as cefotaxime (CTX). This study was aimed to detect the presence of blaCTX-M gene among Pseudomonas aerogenosa (P. aerogenosa) strains isolated from corneal infections in Ibn- Al haitham teaching hospital for eyes in Baghdad. Eleven P. aerogenosa strains were isolated from specimens of corneal infections of patients at Ibn- Al haitham teaching hospital during four months (April- July, 2016).. The results of antibiotic susceptibility of P. aerogenosa strains showed most strains were resist to most antibiotics under test and the highest resistant antibiotic was Cefazolin which gave 11(100%) resistance rate followed by Ampicillin, Aztreonam, Ampicillin-Sulbactam and Amoxicillin-clavulanic acid which gave 9(81.81%) resistance rate. On the other hand the most effective antibiotic under test was Amikacin which it gave the lowest resistance rate 2(18.18%) followed by Chloramphinical which it gave 4(36.36%) resistance rate. Phenotypic detection of ESBL producing strains was done by screening test and the results showed that out of 11 P. aerogenosa, 7(63.64%) were positive ESBL producing strains while 4(36.36%) P. aerogenosa strains were negative ESBL producing strains. Molecular detection of blaCTX-M showed that out of 11 P. aeruginosa strains, 3(27.27%) were harbored the blaCTX gene (544bp) and those 3 strains were also positive ESBL producing strains by phenotypic detection, while 8(72.72%) strains not harbored blaCTX-M gene.
Authors and Affiliations
Dr. Buthainah Mohammed Taha
PREMENSTRUAL SYNDROME- AN ENIGMA TO BEFRIEND:
Introduction: Premenstrual syndrome is a problem experienced by many females of menstrual age group. This menace pesters many women incapacitating some of them to the extent of inability to perform daily activities durin...
USE SIX SIGMA APPROACH TO IMPROVE PATIENT SAFETY AT A GENERAL HOSPITAL
Patient safety is considered the corner stone of the high quality medical care. This study applied Six Sigma set (DMAIC) to identify root cause(s) of medication error and propose appropriate strategies to prevent medicat...
COMPILATION OF HEALTH BENEFITS AND PHYTOCHEMICALS FROM CITRUS PLANTS
Citrus fruits are the main fruit trees grown throughout the world and are well – appreciated for their refreshing juice and health benefits. Numerous therapeutic properties have been attributed to citrus fruits, like ant...
ANATOMICAL VARIATIONS AND THEIR CLINICAL SIGNIFICANCE IN POPULATION OF SOUTHWESTERN AND CENTRAL ETHIOPIA
The concept and contents of the anatomical variations is the history of the anatomy itself. Anatomical variations represent an embryological and comparative background in medicine and biology in order to understand the m...
REVIEW ON SKIN DISEASES, THEIR PATHOLOGY AND TREATMENT
Skin diseases are among the most common health problems worldwide and are associated with a significant burden. The burden of skin disease is a multidimensional concept that Includes psychological, social and financial c...