Molecular detection of Eastern Equine Encephalitis virus in mosquitoes from La Pintada (Antioquia)
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 3
Abstract
Objective. The detection of emerging and re-emerging arboviruses in mosquitoes from urban and rural areas, is fundamental for predict possible epidemic outbreaks in human populations. The Municipality of La Pintada (Antioquia), is characterized by the presence of dry tropical forest relicts, fishing, tourism, farms and mining. An entomological research was performed for explore the possible circulation of arboviruses of public health importance. Materials and methods. Mosquitoes were captured in urban and rural sites in February-April of 2012. The specimens were stored in liquid nitrogens tanks and were grouped using taxonomic keys for genera. RNA extraction from pools and generic/nested RT-PCR was performed for Flavivirus, Alphavirus, Orthobunyavirus (Group Bunyamwera) and Phlebovirus. Results. 1274 mosquitoes were collected, mainly belonging to Culex and Aedes genera. RNA extracts of 64 pools were tested by RT-PCR and one pool was positive for Alphavirus. Sequencing of the RT-PCR product and the analysis with sequences storage in GenBank designate the presence of Eastern equine encephalitis virus (EEEV). Conclusions. This is the first record of natural infection from EEEV in mosquitoes from La Pintada (Antioquia), an area with ecological elements that favor the emergence of emerging and re-emerging arboviruses of medical and veterinarian importance.
Authors and Affiliations
Richard Hoyos L, M. Sc| Universidad de Antioquia, Facultad de Medicina, Grupo de investigación en Medicina Molecular y de Traslación. Carrera 51D Nro. 62-29, Medellín, Colombia.2Universidad Nacional de Colombia – Sede Medellín, Escuela de Biociencias, Grupo de investigación en Sistemática Molecular. Calle 59A Nro. 63-20, Medellín, Colombia. 3Instituto de Salud - Carlos III. Ctra de Majadahonda-Pozuelo Km 2.2, Madrid, España., Juan Suaza V, M. Sc| 2Universidad Nacional de Colombia – Sede Medellín, Escuela de Biociencias, Grupo de investigación en Sistemática Molecular. Calle 59A Nro. 63-20, Medellín, Colombia. 3Instituto de Salud - Carlos III. Ctra de Majadahonda-Pozuelo Km 2.2, Madrid, España., Antonio Tenorio, Ph. D| 2Universidad Nacional de Colombia – Sede Medellín, Escuela de Biociencias, Grupo de investigación en Sistemática Molecular. Calle 59A Nro. 63-20, Medellín, Colombia. 3Instituto de Salud - Carlos III. Ctra de Majadahonda-Pozuelo Km 2.2, Madrid, España., Sandra Uribe, Ph. D| 2Universidad Nacional de Colombia – Sede Medellín, Escuela de Biociencias, Grupo de investigación en Sistemática Molecular. Calle 59A Nro. 63-20, Medellín, Colombia. 3Instituto de Salud - Carlos III. Ctra de Majadahonda-Pozuelo Km 2.2, Madrid, España., Juan Gallego-Gómez, Ph. D *| Universidad de Antioquia, Facultad de Medicina, Grupo de investigación en Medicina Molecular y de Traslación. Carrera 51D Nro. 62-29, Medellín, Colombia.Correspondencia: juanc.gallegomez@gmail.com
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