Molecular Detection of Human Papillomavirus DNA in Oral Lichen Planus Patients
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 1
Abstract
Introduction: Oral Lichen Planus (OLP) is a chronic inflammatory disease with cell mediated immunopathologic response. Even though OLP has been studied widely for decades, the aetiology of the disease is yet not hypothesised exactly. Autoimmunity, viruses like Hepatitis C Virus (HCV), Human Papillomavirus (HPV), Human Immunodeficiency Virus (HIV), Herpes Simplex Virus (HSV), Candida albicans and stress have been hypothesised in recent articles. The fact that there is paucity of studies on the aetiopathogenesis of oral lichen planus pivoted the study to analyse the presence of HPV in OLP. Aim: The aim of this study is to detect the presence of HPV DNA in the biopsy samples of OLP and also to determine the role played by the virus in aetiopathogenesis of OLP. Materials and Methods: Biopsy samples comprising 15 Oral Lichen Planus tissues and 15 normal buccal mucosa tissues embedded in paraffin blocks were collected from different dental colleges located in Telangana erstwhile Andhra Pradesh. Parameters like age, sex, site of lesions, type of lesion were also compared. The DNA was extracted from the fixed tissue by using modified protocol and Polymerase Chain Reaction (PCR) was performed by using general primers to amplify HPV L1 gene. On comparison of sequences of 10 patients by using Basic Local Alignment Search Tool (BLAST), the sequences were found similar to HPV-18 L1 gene. Results: Thirteen out of 15 (86.6%) lichen planus cases and none out of 15 controls were HPV 18 positive. Compared between males and females and controls significant relation was observed between sex and OLP too. However, no significant relation was observed based on the site of the lesion and based on type of lesion. Conclusion: The molecular studies confirm presence of HPV-18 in OLP tissues of 13 patients with 8 Single Nucleotide Polymorphism (SNPs) indicating probably new variant of HPV-18 in Indian patients.
Authors and Affiliations
Ayesha Sameera, Rosaiah Kotikalpudi, Rajesh Kumar Patel, Kranti Kiran Reddy, MD Prasanna, Sridhar Reddy Erugula
Keywords
Related Articles
- EP ID EP556227
- DOI 10.7860/JCDR/2019/32397.12466
- Views 95
- Downloads 0
Comparative Evaluation of Ropivacaine and Fentanyl Versus Ropivacaine and Fentanyl with Clonidine for Postoperative Epidural Analgesia in Total Knee Replacement Surgery
Introduction: Clonidine an alpha 2 adrenoceptor agonist possesses analgesic properties and has been used as an adjuvant in epidural analgesia. The addition of clonidine to other analgesics may result in enhanced analgesi...
The Effect of Cognitive-Behavioural Counselling on Pregnant Women’s Weight Gain during Pregnancy: A Randomised Controlled Clinical Trial
ABSTRACT Introduction: Weight gain during pregnancy has a close relationship with fetomaternal health. An increase in BMI is associated with the risk of premature rupture of membrane, placental abruption and stillbirth....
Pattern of Risk Factors and Correlates of Pruritus among Patients with Chronic Kidney Disease in Sokoto, Nigeria
Introduction: Chronic Kidney Disease (CKD) has become a major public health challenge globally. A cause for concern is the increasing burden of its risk factors, the complications of the disease, and their effects on the...
Ewings Sarcoma of Fourth Metatarsal: A Rare Presentation
ABSTRACT Ewings sarcoma is a primary aggressive neoplasm of bone seen in the second decade of life. This is a case report of a 10-year-old male child who presented with Ewings sarcoma of fourth metatarsal of left side wi...
Prevalence of blaCTX-M, blaCTX-M-2, blaCTX-M-8, blaCTX-M-25 and blaCTX-M-3 Genes in Escherichia coli Isolated from Urinary Tract Infection in Kermanshah City, Iran
ABSTRACT Introduction: Urinary Tract Infection (UTI) is one of the most common bacterial infections and Escherichia coli is the most common organism that causes UTI. However, the incidence of community acquired UTI cause...