Prevalence of blaCTX-M, blaCTX-M-2, blaCTX-M-8, blaCTX-M-25 and blaCTX-M-3 Genes in Escherichia coli Isolated from Urinary Tract Infection in Kermanshah City, Iran
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 8
Abstract
ABSTRACT Introduction: Urinary Tract Infection (UTI) is one of the most common bacterial infections and Escherichia coli is the most common organism that causes UTI. However, the incidence of community acquired UTI caused by Extended spectrum beta-lactamase (ESBL)-producing strains of E.coli, in particular CTX-M genes, is on the rise worldwide. Aim: To detect the frequency of CTX-M gene subgroups in uropathogenic E.coli. Materials and Methods: In this descriptive-analytical study, 240 isolates of E. coli were studied. All isolates were isolated from UTIs in Kermanshah University of Medical Sciences, Kermanshah, Iran, in 2014 to 2015. After screening for ESBL, the CTX-M, CTX-M-2, CTX-M-8, CTX-M-25 and CTX-M-3 genes were detected among ESBL- producing isolates using PCR. Results: Of the 240 E. coli isolates, 67 were ESBL-producing isolates. Sixty one isolates (91%) contained CTX-M gene, of which 57 (85%), 3 (4.5%), 3 (4.5%) and 1(1.49%) contained CTX-M-3, CTX-M-8, CTX- M-25 and CTX-M-2, respectively. Conclusion: Due to the high resistance of E. coli to beta-lactam drugs in this region, these drugs have limited effects for treatment of UTI in outpatient. The frequency of CTX-M-2, CTX-M-8, CTX-M-25 beta-lactamases in isolates of E. coli is relatively low but the overall prevalence of CTX-M and CTX-M-3 beta-lactamases is high which indicates the spread of drug resistance.
Authors and Affiliations
Alisha Akya, Mahnaz Ahmadi, Sepideh Khodamoradi, Mohammad Reza Rezaei, Nahid Karani, Azam Elahi, Roya Chegene Lorestani, Mansour Rezaei
Keywords
Related Articles
- EP ID EP639033
- DOI 10.7860/JCDR/2019/40684.13055
- Views 116
- Downloads 0
Various Clinical and Histopathological Patterns of Idiopathic Photodermatosis: An Observational Study
ABSTRACT Introduction: The idiopathic photodermatosis have different histopathological patterns, spongiotic pattern being the most common. Aim: To study the histopathological patterns of photodermatosis and to correlate...
Knot of Henry: Role in Tendon Transfer
The term “Master knot of Henry” refers to the site of cross connections between long (extrinsic) tendons of foot namely Flexor Digitorum Longus Tendon (FDLT) and Flexor Hallucis Longus Tendon (FHLT). This chiasmatic stru...
Unusual Branching Pattern of the Portal Vein: Its Importance
Portal vein anatomy and its branching pattern are of utmost importance for surgical planning of complex liver resections, living donor liver transplant and radiological procedures. The division of the vein at the porta h...
Incidence of Neurobrucellosis from Central India: A Hospital Based Study
ABSTRACT Introduction: Despite being a rare complication, neurobrucellosis can be a major cause of neurological morbidity and mortality if not adequately diagnosed and treated. Aim: In the present study, we report utilit...
Comparison of Dexamethasone and Ketorolac as an Adjuvant to Bupivacaine in Axillary Brachial Plexus Blocks for Isolated Hand and Forearm Injuries: A Randomised Double-Blind Prospective Study
ABSTRACT Introduction: Brachial plexus nerve blocks for upper limb surgery provide superior analgesia and reduce opioid consumption. Dexamethasone has been an effective adjuvant to local anaesthetics in brachial plexus b...