Molecular Detection of Rubella Virus among Asymptomatic Pregnant Women In Khartoum State
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 3
Abstract
Background: Rubella or ‘German measles’ is transmitted by droplets, direct contact or vertically from pregnant woman to the fetus. The virus is worldwide distributed and of public health importance due to its teratogenic effects. Most infections are asymptomatic and there is no routine screening for rubella among pregnant women. Unfortunately there is no vaccine program in vaccination schedule in Sudan. Aim: This study conducted aimed to detect rubella virus infection by Reverse Transcriptase-PCR among asymptomatic pregnant women. Materials and Methods: One hundred and eighteen (118) blood samples were collected from pregnant women in Khartoum State, plasma were separated and stored till use. RNA was extracted using column based kit and then was converted into cDNA using RT kit. PCR was used to amplify cDNA and gel electrophoresis was done to detect PCR product comparing to DNA ladder. Data were analyzed using SPSS 16.0 by Chi-squire test. Results: Out of 118 plasma samples, 4 (3.4%) specimens were positive by RT-PCR.
Authors and Affiliations
Wafaa Mohammed Abdalla, Shamson khamis khafi, Mohamed Abdelsalam Abdalla
Keywords
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- EP ID EP369949
- DOI 10.9790/0853-1703156468.
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