MOLECULAR IDENTIFICATION OF DERMATOPHYTES AMONG CLINICAL ISOLATES
Journal Title: ASIAN JOURNAL OF NATURAL & APPLIED SCIENCES - Year 2016, Vol 5, Issue 2
Abstract
Aim: This study aimed to identification of dermatophyte species in clinical isolates by both classical and molecular methods, using universal primers for amplification of ITS gene. In addition, identification at a species level was carried out by using PCR-restriction fragment length polymorphism (RFLP) technique. Methodology and Results:In the present study, out of 220 suspected patients (157 females and 63 males) with dermatophytosis were identified by the supervision of specialized dermatologist in the derma unit of the General hospital in Kalar district/Sulaimania province\Iraq during the period from middle of November 2014 to the end of June 2015. Samples were collected from patients included hair fragments, skin scraping and nail clipping which transferred to the research laboratory of Biology Department at Faculty of Education \ University of Garmian, where they immediately examined. Based on the conventional laboratory methods, 80 clinical isolates of dermatophytes showed positive culture which belonging to three genera (Trichophyton, Microsporum and Epidermophyton) and 13 species, , Trichophyton rubrum (downy and granular types) was the most common species 35% followed by T.mentagrophytes 17.5%, M.canis 10% and T.tonsurans 7.5%. Other species T. soudanese; T.interdigitale; T. terrestre represented 5% of dermatophytes while T.concentricum; T.schoenleinii; T.verucosum; Microsporum audouinii; M.gypsium and Epidermophyton flocosum conistitute 2.5%. Molecular identification of dermatophytes was carried by using the primers ITS1 and ITS4 to amplify the internal transcript spacer (ITS) region of ITS1 5.8S ITS2 in rDNA gene, later the species identification was made by digestion of amplified ITS regions by BstN1 restriction enzyme in restriction fragment length polymorphism (PCR-RFLP) to produce distinct band patterns. Conclusions: Survey of dermatophytes showed that PCR-RFLP is a rapid and easy method for dermatophytes identification; therefore we suggest using the PCR-RFLP corresponding to the conventional laboratory identification including macroscopic and microscopic examination.
Authors and Affiliations
Khalid A. Habeb, Hana K. Maikhan, Shwan K. Rachid
Keywords
Related Articles
- EP ID EP204394
- DOI -
- Views 84
- Downloads 0
THE CHANGES IN THE UTERUS OF OVAREICTOMIZED MICE IN RESPONSE TO PLUMERIA ACUMINATA AIT STEM EXTRACT
Objective: The primary objective of this study is to assess the effect of methanolic stem extract of Plumeria acuminata Ait. on the uterus and estrogenic/antiestrogenic potency of the plant, which are traditionally used...
THE EFFECT OF INVOLVING MORE DERIVATIVE PROPERTIES, ON THE ACCURACY OF AN IMPLICIT ONE-STEP METHOD
This work describes the development of a class of implicit one-step multi-derivative methods of the form; n j i j ij j l i i j n j y h β y + = = = ∑ ∑ + = ∑ 1 0 1 0 1 α ’ 1 α1 = + with lo...
SYNTHESIS AND CHARCTERIZATION OF 2-BENZOXAZOLONE AND ITS DERIVATIVES
2(3H)-Benzoxazolone derivatives are compounds that possesses different biological activity particularly analgesic and anti-inflammatory activities according to the literature, in this research two benzoxazolone derivativ...
Ground Roll Suppression using Wigner Distribution
A data processing strategy to suppress seismic noises by Wigner Distribution (WD) method through signal decomposition in time-frequency domain is demonstrated. The 48 WDs are used for a profile with 48 channel vertical c...
HIGH ORDER A-STABLE BLOCK ETR2S AND THEIR APPLICATION TO SYSTEM OF FIRST ORDER ODES
An eighth order block Extended Trapezoidal Rule of Second kind (ETR2s) is presented for the numerical integration of stiff system of first-order ordinary differential equations. In the derivation process, we adopt the po...