Molecular Identification of Mediterranean Mutation in Patients with Deficiency of Glucose-6-Phosphate Dehydrogenase (G6pd) in North West of Iran

Journal Title: Journal of Physiology and Pharmacology Advances - Year 2014, Vol 4, Issue 7

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) is a highly polymorphic enzyme that is encoded by human X-linked gene.G6PD deficiency is the most common enzymopathy in human with approximately 400 million people affected worldwide. According to the previous studies, Mediterranean mutation (C563T) is one of the most common G6PD enzyme mutations in Iranian population with a prevalence range of %63 to %86/4.This study was conducted in order to determine Mediterranean mutation, through molecular method ,in the population of the North-west of Iran. In this study, by Rapid Genomic DNA Extraction (RGDE) method, from 77 blood samples of unrelated male and female patients with genetic deficiency of G6PD, DNA was extracted and after digestion by MboII enzymes, in order to search for Mediterranean mutation, they were analyzed by means of PCR-RFLP and sequencing methods. Of the 77 patients, 48 patients (37 male, 11 female) had a Mediterranean mutation frequency of 62/3 %. 29 patients (37/7%) had mutations other than Mediterranean type. This study revealed that G6PD is the most common mutation in Iranian North-west population. Further studies are recommended to identify the mutation type of other varieties.

Authors and Affiliations

M. Valizadeh , H. Onsori , A. Fathi , B. M. Jabbarpour , F. Amani

Keywords

Related Articles

The Effect of Topical Honey on the Healing of Cornea Following Alkaline Injury and Surgical Abrasions in Rats

Corneal traumatic injury and chemical burn are common types of corneal injury that may lead to persistent ulceration, corneal opacification and angiogenesis. This study aims to assess the therapeutic effects and the unde...

In vitro Hemostatic Activity Screening of Sap of Jatropha Multifida L. (Euphorbiaceae) used in Traditional Medicine at Cotonou (Benin)

This study has evaluated the hemostatic potential of Jatropha multifida’s sap as traditional medicine in Benin attributed hemostatic properties to this substance. Several hemostatic tests as Blood Coagulation Time, Quick...

A Case Control Study to Assess Impact of Risk Factors on Trends of Lung Cancer

Lung cancer is a leading cause of death with changing trends of occurrence, detection and treatment. The objective of this research was to identify impact of risk factors on changing trends of lung cancer in a case contr...

Cytokines and Hepatic Evaluation in Wistar Rats Treated with NSAIDs

The present study aims to evaluate hepatic histology and quantify serum dosage of cytokines on an experimental trial with the non-steroidal anti-inflammatory drugs, diclofenac, meloxicam and firocoxib. Ninety Wistar rats...

Pranayama Has Additive Beneficial Effects Along with Medication in Bronchial Asthma Patients

Bronchial asthma is a large economic burden i.e. 12.7 billion dollar globally. Bronchial asthma is the most common cause of missed school days in children i.e. 10.2 million & 3 million missed working days for adults...

Download PDF file
  • EP ID EP99900
  • DOI -
  • Views 107
  • Downloads 0

How To Cite

M. Valizadeh, H. Onsori, A. Fathi, B. M. Jabbarpour, F. Amani (2014). Molecular Identification of Mediterranean Mutation in Patients with Deficiency of Glucose-6-Phosphate Dehydrogenase (G6pd) in North West of Iran. Journal of Physiology and Pharmacology Advances, 4(7), 389-395. https://europub.co.uk/articles/-A-99900