MOLECULAR IDENTIFICATION OF PHYTOPLASMA ASSOCIATED WITH SOYBEAN WITCHES-BROOM IN VIDARBHA REGION, MAHARASHTRA
Journal Title: International Journal of Agriculture Sciences - Year 2017, Vol 9, Issue 21
Abstract
Soybean (Glycine max L.) plants showing witches’-broom symptoms typical of phytoplamas were observed from different locations of Vidarbha region, Maharashtra. Symptoms observed under field condition include phyllody, stunting growth, proliferation of auxiliary shoots resulting in a witches-broom appearance and deformed pods if any. PCR detection of soybean phytoplasma by using phytoplasma-specific universal primer pairs (P1/P7) produced an amplified product of 1.8kb lengths when DNA extract from symptomatic samples was used as template. Asymptomatic plant samples yielded no amplification. On mechanical inoculation none of the test plants inoculated showed any visible symptoms even after 120 days after inoculation
Authors and Affiliations
M. HAJONG, P. C. ATRAM, S. S. MANE
Keywords
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