Molecular Study of Glycoprotein (G) Gene Region of Rabies Virus from Spotted Deer, Delhi, India
Journal Title: Journal of Communicable Diseases - Year 2018, Vol 50, Issue 3
Abstract
Rabies is a viral zoonotic disease of public health importance in India. The disease is usually transmitted by a rabid animal bite, scratches and licks on broken skin and mucous membrane. Rabies is reported from all continents except Antarctica although prevalence is more in Asia and Africa. India is endemic for rabies virus (RABV) throughout the country except for islands of Lakshadweep, Andaman & Nicobar. In the present study, brain samples of clinically suspected animals obtained during the RABV outbreak at National Zoological Park, New Delhi, India, in 2016 were initially tested for rabies antigen by DFAT. All the 32 DFAT positive brain samples were then processed for RNA isolation. The extracted RNA was amplified for glycoprotein (G) gene of 590 bp by RT-PCR followed by nucleotide sequencing. Nucleotide alignment revealed only substitutions without any insertion or deletion. Amino acid alignment revealed replacement at four positions namely L244I, T264A, M310I and N375K. The amino acid change at position where Leucine is replaced by Isoleucine has not been observed in available RABV references of the G gene region either from India or other countries. This mutation was observed in all the samples included in the present study. A phylogenetic tree showed that all the study sequences belonged to Arctic like 1a lineage, in RABV genotype 1. In conclusion, RABV in the study region is acquiring genetic variation; which may have effect on the pathogenicity. This indicates that continued molecular surveillance of the circulating rabies virus strains should be carried out.
Authors and Affiliations
Dr. M Chhabra,
Keywords
Related Articles
- EP ID EP404872
- DOI 10.24321/0019.5138.201817
- Views 123
- Downloads 0
Isolation and Current Trend in Antimicrobial Resistance for Shigella in Diarrhea Patients in New Delhi: Hospital-Based Study
Introduction: Shigella species, members of the family Enterobacteriaceae, are responsible for causing acute gastroenteritis which is one of the most common causes of morbidity and mortality in children in developing coun...
Vibrio Cholerae Outbreak in Batala Town, Punjab, India 2012
Background: On 4th October 2012, a death due to ADD was reported at Civil Hospital Batala, Punjab followed by a sudden rise in the number of cases. We sought to establish the cause and source of infection. Methods: A ca...
Assessment of Knowledge, Attitude and Practices in different Socio-Economic Groups of Population on Control of Dengue and its Vectors in Delhi
Active involvement of community in dengue control is a function of the knowledge and practice of that community. Correct assessment of community attitude, knowledge and practice can assist the reformulation of dengue con...
Modified Ovitrap to Control Aedes Sp Population in Central Java, Indonesia
Ovitrap has been developed and used in many countries as a monitoring tool of dengue vector. The aim of this study was to compare the effectiveness of modified ovitrap and standard ovitrap, as well as measuring the popul...
Dengue Vector Surveillance in and around Mormugao Port Trust (MPT) – Goa, India
As per the International Health Regulations, it is a moral responsibility on national governments to keep international seaports/airports and peripheral areas up to 400 meters free of vector mosquitoes in its adult and i...