Molecular Typing with PFGE Method of Methicillin-resistant Staphylococcus aureus Isolates from in a University Hospital
Journal Title: Hippocrates Medical Journal - Year 2021, Vol 1, Issue 1
Abstract
Objectives: Staphylococcus aureus is a pathogen that causes many infections in humans, and the emergence of methicillin-resistant (MRSA) isolates in the last fifty years poses a threat to society and hospitals. Preventing the spread of MRSA strains between and within hospitals can only be achieved with a correct typing. The PFGE method is considered to be the gold standard among molecular typing methods. Methods: In this study, a clonal relationship between nine methicillin-resistant S. aureus isolates isolated from Van Yüzüncü Yıl University hospital was determined .Pfge analysis was done with the Bionumerics program. The similarity index was created at a tolerance value of 1% using the “Dice” coefficient. The clustering relationship between isolates was demonstrated using the UPGMA (unweighted pair group method of arithmatic averages) method. The principles set out by Tenover et al. were adopted for the evaluation of genotypic association between isolates Results: As a result of the analysis 4 independent genotypes were identified. It was determined that there were 2 related isolates in 3 genotypes. Conclusion: In our study, it was determined that there was not a dominant MRSA clone in different years in the hospital studied, since samples from different years were studied. However, it is thought that it would be beneficial to increase the precautions in order to prevent the distribution of infection in all hospitals and to control the infection in all areas.
Authors and Affiliations
Sümeyra SAVAŞ, Mehmet PARLAK
Keywords
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- EP ID EP698836
- DOI https://doi.org/10.29228/HMJ.3
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