Abstract

Objective: To describe the morphological and molecular characterization of a newly isolated Gordius spp. from of the northeastern states of India. Methods: Identification of the parasite was based on light microscopy and scanning electron microscopy. For molecular identification a partial sequence of ITS2 region was amplified. The product was cloned in cloning vector and subsequently phylogenetic analysis was done. Results: Based on the morphological and molecular analysis, the nematode infesting cricket was confirmed to be Gordius species. Grossly the length of those nematodes ranged between 15.1 and 35.5 cm. The present study also described the light microscopy and fine structural morphological study of the isolated species. Molecular amplification targeting ITS2 region of Gordius was found to be approximately 1 048 bp. Phylogenetic analysis revealed that the Gordius species is a close relative (100%) to Malaysian species. Conclusions: Occurrence of Gordius species in Indian cricket has been confirmed and found 100% similarity with Malaysian species.

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