Morphological and SSR Assessment of Putative Drought Tolerant M3 and F3 Families of Wheat (Triticum aestivum L.)
Journal Title: Biotechnology Journal International - Year 2015, Vol 6, Issue 4
Abstract
In an attempt to develop drought tolerant genotypes of bread wheat, two procedures, i.e., mutation and hybridization were used to induce new genetic variation. Selection for high grain yield/plant (GYPP) and other desirable traits was practiced in the M2 populations of 7 gamma irradiated genotypes and F2 populations of 15 diallel crosses among 6 genotypes of wheat under well watering (WW) and water stress (WS) conditions. Progenies of these selections (53 M3 and 109 F3 families) and their seven parents were evaluated in the field under WW and WS. Significant yield superiority of twelve families (7 M3’s and 5 F2’s) over their original and better parents, respectively under WS reached 74.71% (SF9). These putative drought tolerant families were assessed on the DNA level using SSR analysis. Fifteen SSR primers were used for PCR amplification of the genomic DNA of these 12 selections and their parents. The SSR analysis proved that the 12 families are genetically different from their 7 parents, with an average polymorphism of 86.67%. The genetic similarities (Gs) ranged from 30% to 88%. Both mutants SF3 and SF4 exhibited very low Gs (42 and 40%, respectively) with their common parent (Giza-168), indicating that gamma rays were very effective in changing the genetic background of Giza-168 towards high GYPP under WS conditions. SSR assay permitted the identification of seven unique bands (5 positive and 2 negative) for three drought tolerant wheat genotypes (SF3, SF4 and Aseel-5). These bands might be considered useful as markers associated with drought tolerance in bread wheat breeding programs.
Authors and Affiliations
A. M. M. Al-Naggar, Kh. F. Al-Azab, S. E. S. Sobieh, M. M. M. Atta
Keywords
Related Articles
- EP ID EP237133
- DOI 10.9734/BBJ/2015/15898
- Views 128
- Downloads 0
Effects of Exogenous Salicylic Acid on the Antioxidative System in Bean Seedling Treated with Manganese
In the present study we investigated the role of salicylic acid (SA) in regulating Mn-induced oxidative stress in bean (Phaseolus vulgaris) leaves. Exposure of plants to 100 µM Mn inhibited biomass production and intensi...
Computational Molecular Analysis of the Sequences of PAPPA2 Gene of Selected Ruminants and Non Ruminants
Pregnancy associated plasma protein A2 (PAPPA2) is an Insulin-like growth factor binding protein (IGFBP) protease of the pappalysin family. This gene has been reported to be associated with prenatal growth, postnatal gro...
Heavy Metals Concentration in Fish Mugil cephalus from Machilipatnam Coast and Possible Health Risks to Fish Consumers
Heavy metals are dangerous to aquatic organisms and it can be bioaccumulated in the food chain leading to diseases in humans. Cumulative effects of metals or chronic poisoning may occur as a result of long term expore ev...
Assessment of Antiproliferative Potential of Hexalobus crispiflorus (Annonaceae)
Background: Hexalobus crispiflorus root aqueous extract (HC) is used to treat breast cancer in Nigeria. Aim: To evaluate the antiproliferative effects of HC using Sorghum bicolor seed radicle as test subject. Materials...
Detection and Analysis of the Random Mutagenesis Site(s) of Xylanase Gene from Mutants of Bacillus subtilis subsp. spizizenii ATCC 6633
Aims: A total of five mutant strains of Bacillus subtilis subsp. spizizenii ATCC 6633 designated as the MXB 1, MXB 2, MXB 3, MXB 4 and MXB 5 were developed using random mutagenesis of ethyl methane sulfonate (EMS) and ac...