Multiple Shoots Induction from Indigenous Nigerian Pumpkin (Cucurbita pepo L.)
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 17, Issue 5
Abstract
Aim: The aim of this study was to develop a protocol for in vitro regeneration of a Nigerian indigenous pumpkin (C. pepo L.) via seedling-derived cotelydon, cotyledonary node and hypocotyl explants. Study Design: A combination of 0.00, 1.00, 2.00 and 3.00 mgl-1 of 6-Benzylaminopurine (BAP) and 0.00 or 0.05 mgl-1 of 2,4-Dichlorophenoxy acetic acid (2,4-D) for each explant type were set up in three replicates making a total of 36 culture vessels in the entire experimental set up. Five explants were cultured per combination. Place and Duration of Study: The work was conducted in the Plant Tissue Culture Laboratory of the Department of Biological Sciences, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria, between January 2017 and July 2017. Methodology: Hypocotyl, cotyledonary node and cotyledon explants derived from 4-week old-seedlings were cultured on MS medium fortified with 0.00, 1.00, 2.00 and 3.00 mgl-1 of BAP in combination with 0.00 or 0.05 mgl-1 of 2,4-D and investigated for callus, shoot and root induction. Results: A combination of 1.00 mgl-1 BAP with 0.05 mgl-1 2,4-D was optimum for callus induction from hypocotyl and cotyledonary node explants, while for cotyledon explants, 2.00 mgl-1 BAP in combination with 0.05 mgl-1 2,4-D was preferred. Cotyledonary node explants and cotyledonary node explant-derived callus responded with multiple shoots (4.50±0.042 and 4.07±0.067 shoots per explant, respectively) on full strength Murashige and Skoog (MS) medium (control) devoid of Plant Growth Regulators (PGRs). When the different explant types were cultured on MS media amended with the different concentrations of BAP in combination with 2,4-D, neither shoot nor root induction was observed. All the explants initiated roots when cultured on full strength PGR-free medium. Only cotyledon explant-derived callus formed roots (2.30±0.56cm) on PGR-free MS medium. Conclusion: Regeneration of indigenous vegetables, such as Cucurbita pepo is achievable through hypocotyl, cotyledonary node and cotyledon explants with or without PGRs.
Authors and Affiliations
Olawole O. Obembe, Oluwadurotimi S. Aworunse, Oluwakemi A. Bello, Abosede O. Ani
Keywords
Related Articles
- EP ID EP313439
- DOI 10.9734/ARRB/2017/35756
- Views 72
- Downloads 0
Molecular Genetic Markers for Assessing the Genetic Variation and Relationships in Lactuca Germplasm
The genus Lactuca L. belonging to the Asteraceae family, is widely distributed in different geographical and ecological areas. Lettuce and most of the other species of the genus Lactuca L. have been cultivated for their...
Effectiveness of Ethyl Acetate Extract of Endophytic Fungi in Soursop Leaves towards the Growth of Mammary Tumor Induced by 7,12-dimethylbenz(α)anthracene in Female Rats
Aims: This research aims to investigate the effectiveness of ethyl acetate extract of endophytic fungi isolated Sir G5 from soursop leaf (Annona muricata L.) in vivo breast tumor growth using Sprague-dawley (SD) female s...
Cadmium Induced Toxicity in Human Peripheral Blood Lymphocytes Culture and Its Amelioration by Vitamin C
Introduction: Cadmium is a ubiquitous and toxic environmental pollutant increasing worldwide due to high consumption in various industries. At the cellular level, cadmium affects cell proliferation, differentiation, apop...
Nonglycine Residues in Proteins should most likely have an Allowed Conformation with a Negative Value for Backbone Torsion Angle f
Backbone torsion angles , of a vast majority of nonglycine residues in proteins lay within the peculiar insulated regions in the (,) space assigned to the conformations allowed for nonglycine residues that embrace t...
Evaluation of the Salinity Stress Effect on Cumin (Cuminum cyminum L.) Ecotypes in Kerman, Iran
Cumin (Cuminum cyminum L.) is one of the most important Medicinal plants. Since many semi arid and arid areas of Iran are affected by salinity and accessing water resources has been decreased. Proper measures need to be...