Musculoskeletal Trauma Treated With Bone Marrow Mesenchymal Stem Cells in Wistar Albino Rat
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2015, Vol 3, Issue 2
Abstract
Stem cell research could lead to a new bone repair therapy in musculoskeletal trauma (MST). Regenerative medicine attempts to restore living tissue, which has been lost or damaged. To generate bone cells for regenerative medicine remains a significant challenge. Bone marrow mesenchymal stem cells (BMSCs) harvested from wistar albino rat was transplanted into traumatized site. It is highly interdisciplinary field, which involves isolation, characterization, differentiation and transplantation of bone marrow mesenchymal stem cells with CT and Histopathology observations. In this study 9 male and 9 female rat with age above one year and weight between 150 to 250 grams were required for the study. The animals were divided in to 3 groups (6 each.) group-A -MST Control, group-B -MST treated with Stem cells, group-C -MST treated with Stem cells and PLGA scaffold. CT was taken on 3rd, 10th & 30th day to confirm the progress of healing. When compared the control A with group C there is a statistical significance in callus formation in 3rd day(p<.01), 10th day(p<.01), and 30th day(p<.01). At the end of 30th day the histopathology observation confirms with computer tomography observations. Autologous intravenous/bone marrow mesenchymal stem cell therapy favours’ faster and complete healing in musculoskeletal injuries. Keywords: Musculoskeletal trauma (MST), Bone marrow mesenchymal stem cells (BMSCs), Fluorescence activated cell sorting (FACS), Poly lactic co-glycolic acid (PLGA).
Authors and Affiliations
P. Saraswathi, K. Balaji, Julie Christy, S. Saraavanakumar, R. Vijayaraghavan
Keywords
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