Natural Incidence and Genetic Variability of Erysiphe pisi, the Causal Agent of Powdery Mildew on Peas in the Nilgiris District, Tamil Nadu, India

Journal Title: Current Journal of Applied Science and Technology - Year 2017, Vol 24, Issue 5

Abstract

Erysiphe pisi, which causes pea powdery mildew, is an obligate biotrophic pathogen that can easily adapt to its host plant. Understanding the natural incidence and genetic variations of the pathogen is essential for disease control and for breeding resistant variety to pea powdery mildew. The incidence was studied in different locations of the Nilgiris district, and the disease recorded from 32.61 to 45.55 and 14.08 to 20.84 per cent in leaves and pods, respectively. A total of 10 isolates of E. pisi were obtained from the diseased samples to study the genetic variability if any, among them using internal transcribed spacer region amplification and inter-simple sequence repeats (ISSRs). PCR amplification of total genomic DNA with ISSR primers generated unique banding patterns depending upon primers and isolates. Twelve oligonucleotide primers were selected for the ISSR assays, which resulted in 742 bands for ten isolates of E. pisi. The number of bands obtained was entered into a PRIMER 7 to understand genetic relationship and Bray-Curtis coefficient for hierarchical cluster analysis. The results showed that the variability among the pathogen isolates was moderate. The above ten isolates were grouped into two major clusters and one single cluster had an average Polymorphic Information Content value (PIC) of 0.883 and Effective Multiplication Ratio (EMR) of 90. Hence, it is inferred that the E. pisi infecting peas in the Nilgiris consists of a single clonal lineage with a moderate level of genetic diversification.

Authors and Affiliations

S. Parthasarathy, M. Muthamilan, D. Alice, T. Raguchander

Keywords

Related Articles

Optimization of the Rehabilitation of Concrete Buildings Based on Their Dynamic Properties

The present work describes the development of an optimization model for the rehabilitation process of concrete buildings damaged mainly by the effects of an earthquake. Currently, the process is carried out according to...

Measurement and Determination of Emission Levels of Ionizing Radiation from High Tension Lines around Kaduna Metropolis, North West, Nigeria

Gamma radiation dose rate was measured at the base of 11 KV, 33 KV, 132 KV and 330 KV high tension lines (power lines) from different locations in Kaduna including Mando transmission sub-station using Radex 1503+ model....

Assessing of Farmers’ Opinion towards Floating Agriculture as a Means of Cleaner Production: A Case of Barisal District, Bangladesh

Aims: Bangladesh, as a low-lying country, is vulnerable to global climate change and affected by floods and water logging. Hence, the country needs to adopt sufficient adaptation strategies which are based on local peopl...

Investigating Effect of Zinc Content on the Mechanical and Corrosion Responses of Al6063-SiC Composite

Corrosion susceptibility of Al6063-SiC based composites is attracting unceasing concerns among researchers. The motive of this work was to investigate the influence of zinc content on the corrosion resistance and mechani...

Design and Construction of a Solar Water Heater for Environmental Sustainability

This research work involves the design and construction of a solar water heating system with 2.3 m2 solar collector area proposed to extract enough solar energy to raise the temperature of 0.075 m3 of water by...

Download PDF file
  • EP ID EP319133
  • DOI 10.9734/CJAST/2017/37933
  • Views 85
  • Downloads 0

How To Cite

S. Parthasarathy, M. Muthamilan, D. Alice, T. Raguchander (2017). Natural Incidence and Genetic Variability of Erysiphe pisi, the Causal Agent of Powdery Mildew on Peas in the Nilgiris District, Tamil Nadu, India. Current Journal of Applied Science and Technology, 24(5), 1-11. https://europub.co.uk/articles/-A-319133