Natural versus synthetic fixative in oral cytological smears – A double blind study
Journal Title: Indian Journal of Pathology and Oncology - Year 2018, Vol 5, Issue 4
Abstract
ObjectiveFixation in cytology is to preserve and maintain clear and consistent morphological features Ethanol is most commonly used a gold standard fixative in cytology but ithas few disadvantages such as expensive flammable evaporates easily subjected to pilferage and not easily availableSo honey can be used as fixative as it contains several minerals trace elements vitamins carbohydrates and acts as a fixative because of itsinherent properties by enabling cellular preservation and dehydration Considering this objective of present study is to evaluate the fixative properties of honey in comparison with ethanol in a double blinded mannerMaterials and MethodsTwooral smears were obtained from each individual n50 by gently scraping the buccal mucosa One slide was fixed in ethanol 95 and the other in honey 20 processed for a minimum of 15 minutes and stained with Papanicolaou stain Two separate pathologists who were blinded for the fixative used evaluated the slides for 5 parameters Cell morphology Nuclear and Cytoplasmic staining Clarity and uniformity of stainingResultsAmong 50 cases evaluated by both pathologists results showed that honey fixed smears had acceptable nuclear and cytoplasmic staining well preserved cell morphology clarity and uniformity of staining as comparable to ethanol with no statistical difference between both fixativesConclusionHence in search of a better ecofriendly and cost effective fixative20 processed honey can be efficiently used in cytological smear fixation for the preservation of cellular detailsKeywords Buccal smear Ethanol Fixation
Authors and Affiliations
Savitri M Nerune, Mahmood Nawaz Khan, R M Potekar, Vijayalaxmi Patil
Keywords
Related Articles
- EP ID EP475950
- DOI 10.18231/2394-6792.2018.0126
- Views 34
- Downloads 0
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