A REVIEW ON CURRENT TRENDS AND ADVANCEMENTS IN BIOANALYTICAL METHODS
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 6
Abstract
ABSTRACT This paper reviews the recent developments in bio-analysis sample preparation techniques and chromatographic techniques give an update on basic principles and a comparative discussion on the benefits and limitation of every technique. Any bio-analytical technique includes several steps, all of them being vital in order to attain reliable results. The first step is taking aliquots of samples for the analysis, followed by the extraction procedure and sample clean-up, chromatographic analysis and detection. Conventional sample preparation techniques including solid phase extraction (SPE), liquid-liquid extraction (LLE), protein precipitation (PP) and many modern approaches including molecularly imprinted polymers (MIP), solid phase micro extraction (SPME), liquid-liquid micro extraction (LLME), micro extraction by packed sorbent (MEPS) and plenty of others have additionally been featured as fundamental and significant step of bio-analytical methods. Current trends in fast liquid chromatographic separations involve monolith technology, high temperature liquid chromatography (HTLC) and ultra-high performance liquid chromatography (UHPLC). UHPLC has recently become a wide-spread analytical technique in many laboratories which focus on fast and sensitive bio-analytical assays. The key advantages of UHPLC are the increased speed of analysis, higher separation efficiency and resolution, higher sensitivity and much lower solvent consumption as compared to other analytical approaches. This is all enabled by specially designed instruments and sub-2-microne particle packed analytical columns. Keywords: Bio-analytical, Solid phase extraction (SPE), Liquid liquid extraction (LLE), Protein precipitation (PP), Micro dialysis, Micro extraction, Molecularly imprinted polymer (MIP), Micro extraction in packed syringe (MEPS), Monolith, HILIC, UHPLC.
Authors and Affiliations
Vidya K*, Uma Maheshwara Rao V, Ajitha A
Keywords
Related Articles
- EP ID EP143895
- DOI -
- Views 116
- Downloads 0
STABILITY INDICATING CHROMATOGRAPHIC METHODS FOR ACTIVE PHARMACEUTICAL INGREDIENTS AND FORMULATIONS – A REVIEW
ABSTRACT The article highlights on the development of validated stability indicating methods for active pharmaceutical ingredients and formulations. International Council on Harmonization (ICH) prescribed differe...
A VALIDATED SIMULTANEOUS ESTIMATION OF DOXYLAMINE SUCCINATE AND PYRIDOXINE HYDROCHLORIDE BY UV – SPECTROPHOTOMETRIC METHOD IN BULK AND FORMULATION
ABSTRACT A simple and rapid UV – Spectrophotometric method was developed and validated for the simultaneous estimation of Doxylamine succinate and Pyridoxine HCl in combined dosage forms. The said combination is...
ESTIMATION OF LAMIVUDINE IN BULK AND FORMULATION BY TITRIMETRIC AND UV-VISIBLE SPECTROPHOTOMETRY
ABSTRACT Lamivudine is a synthetic chemotherapeutic agent used to treat HIV infection and hepatitis-B viral infection by inhibiting viral reverse transcriptase enzyme. So is very important to estimate the dosage...
ANTIOXIDANT DEFENSE ENZYMES ACTIVITY IN HYPOBARIC HYPOXIA SUSCEPTIBLE AND TOLERANT SPRAGUE DAWLEY RATS
ABSTRACT Antioxidant enzymes are known defense molecules, which eliminate reactive oxygen species (ROS) and free radicals that damage useful enzymes in the cell under various stress conditions. Significant effort...
AN OVERVIEW ON FLOWCYTOMETRY
ABSTRACT Flowcytometry is a powerful technology mainly used for investigations in cell biology and isolation of individual cells.It is a laser based biophysical analytical technique used mainly for cell sorting,...