A REVIEW ON CURRENT TRENDS AND ADVANCEMENTS IN BIOANALYTICAL METHODS
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 6
Abstract
ABSTRACT This paper reviews the recent developments in bio-analysis sample preparation techniques and chromatographic techniques give an update on basic principles and a comparative discussion on the benefits and limitation of every technique. Any bio-analytical technique includes several steps, all of them being vital in order to attain reliable results. The first step is taking aliquots of samples for the analysis, followed by the extraction procedure and sample clean-up, chromatographic analysis and detection. Conventional sample preparation techniques including solid phase extraction (SPE), liquid-liquid extraction (LLE), protein precipitation (PP) and many modern approaches including molecularly imprinted polymers (MIP), solid phase micro extraction (SPME), liquid-liquid micro extraction (LLME), micro extraction by packed sorbent (MEPS) and plenty of others have additionally been featured as fundamental and significant step of bio-analytical methods. Current trends in fast liquid chromatographic separations involve monolith technology, high temperature liquid chromatography (HTLC) and ultra-high performance liquid chromatography (UHPLC). UHPLC has recently become a wide-spread analytical technique in many laboratories which focus on fast and sensitive bio-analytical assays. The key advantages of UHPLC are the increased speed of analysis, higher separation efficiency and resolution, higher sensitivity and much lower solvent consumption as compared to other analytical approaches. This is all enabled by specially designed instruments and sub-2-microne particle packed analytical columns. Keywords: Bio-analytical, Solid phase extraction (SPE), Liquid liquid extraction (LLE), Protein precipitation (PP), Micro dialysis, Micro extraction, Molecularly imprinted polymer (MIP), Micro extraction in packed syringe (MEPS), Monolith, HILIC, UHPLC.
Authors and Affiliations
Vidya K*, Uma Maheshwara Rao V, Ajitha A
Keywords
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