MICELLAR ELECTROKINETIC CHROMATOGRAPHY: AN OVERVIEW
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 4
Abstract
ABSTRACT Micellar electrokinetic chromatography (MEKC) is a useful branch of capillary electrophoresis (CE) that utilizes surfactant above critical micellar concentration (CMC) as pseudo-stationary phase. MEKC can be employed to separate both charged and neutral molecules, individually or simultaneously, including chiral compounds. MECK benefits from high peak efficiency due to electroosmotic flow (EOF) in the separation capillary, compounded with large variety of synthetic surfactants, organic modifiers, temperature and variable separation voltage has made MECK. In this review, we present the introduction of CE, fundamentals of surfactant chemistry as it relates to MEKC, separation principles in MECK including equations involved in calculating separation parameters. Keywords: Micelles, Surfactants, Buffer additives, Optimization parameters.
Authors and Affiliations
T. Manjula* , G. Nagasowjanya, A. Aitha, V. Uma maheswara Rao
Keywords
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