MICELLAR ELECTROKINETIC CHROMATOGRAPHY: AN OVERVIEW
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 4
Abstract
ABSTRACT Micellar electrokinetic chromatography (MEKC) is a useful branch of capillary electrophoresis (CE) that utilizes surfactant above critical micellar concentration (CMC) as pseudo-stationary phase. MEKC can be employed to separate both charged and neutral molecules, individually or simultaneously, including chiral compounds. MECK benefits from high peak efficiency due to electroosmotic flow (EOF) in the separation capillary, compounded with large variety of synthetic surfactants, organic modifiers, temperature and variable separation voltage has made MECK. In this review, we present the introduction of CE, fundamentals of surfactant chemistry as it relates to MEKC, separation principles in MECK including equations involved in calculating separation parameters. Keywords: Micelles, Surfactants, Buffer additives, Optimization parameters.
Authors and Affiliations
T. Manjula* , G. Nagasowjanya, A. Aitha, V. Uma maheswara Rao
Keywords
Related Articles
- EP ID EP133509
- DOI -
- Views 122
- Downloads 0
DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF CEFTAROLINE FOSAMIL IN BULK AND INJECTION FORM
ABSTRACT Two simple, sensitive, specific, spectrophotometric methods have been developed for the determination of ceftaroline fosamil in bulk and Injection forms. The λmax of ceftaroline fosamil was found to be 2...
ELECTROCHEMICAL REDUCTION BEHAVIOUR AND DETERMINATION OF CEFQUINOME
ABSTRACT The electrochemical reduction behaviour of cefquinome has been studied by using dc polarography, cyclicvoltammetry, ac polarography, differential pulse polarography, millicolommetry and controlled poten...
AN OVERVIEW ON FOURIER TRANSFORM MASS SPECTROMETRY
ABSTRACT This article provides an introduction to Fourier transform-based mass spectrometry. The key performance characteristics of Fourier transform-based mass spectrometry, mass accuracy and resolution, are prese...
SUPER CRITICAL FLUID CHROMATOGRAPHY: AN OVERVIEW
ABSTRACT Supercritical Fluid Chromatography (SFC) is a form of normal phase chromatography, first used in 1962.SFC typically utilizes carbon dioxide as the mobile phase; therefore the entire chromatographic flow...
DEVELOPMENT AND VALIDATION OF NEW SPECTROPHOTOMETRIC METHODS FOR THE QUANTITATIVE ESTIMATION OF SILODOSIN IN BULK DRUG AND PHARMACEUTICAL FORMULATIONS
ABSTRACT Three simple, sensitive, accurate and rapid spectrophotometric methods A, B and C have been developed for the quantitative estimation of Silodosin in bulk drug and also pharmaceutical formulations. Metho...