A REVIEW ON TIME CORRELATED SINGLE PHOTON COUNTING
Journal Title: Int J of Preclin & Pharm Res - Year 2014, Vol 4, Issue 4
Abstract
ABSTRACT The phenomenon of molecular fluorescence emission contains both spectral and time information it is former which has traditionally been more usually associated with routine analytical applications of fluorescence spectroscopy. In contrast, since the early seventies, the most important strides in fluorescence spectroscopy have been by and large concerned with the development and application of new time resolved techniques. Applications of these now span not only in the traditional areas of photochemistry but also research in polymers, membranes, liquid crystals, low-dimensional structures, solar energy, and semiconductors, to name but a few. Such diverse areas are linked by their common instrumentation needs, which have greatly benefited over the past decade from quite radical progress in optoelectronics and on-line data analysis. There is now widespread recognition of the advantages of studying and using fluorescence in the time domain. Quite simply, fluorescence measurements in the time domain (fluorometry) posses a much greater information content about the rates and hence kinetics of intra and intermolecular processes than is afforded by the wavelength spectroscopy of the energy domain (fluorimetry). A simple analogy to the greater detail shown by time domain as compared to steady state fluorescence measurements is that of motion pictures in comparision to still photographs. The rising importance of fluorescence measurements in the time domain has been reflected during the past decade in several texts devoted exclusively to this topic. Nevertheless, the field has not stood still, and, if anything, the rate of innovation has probably increased over the past few years. Keywords: Time correlated single photon counting, Fluorescence lifetime, Photon signals, Luminescence.
Authors and Affiliations
Vineela P*, Vivek Sagar, Pavani B, Ajitha A, Uma Maheshwara Rao. V
Keywords
Related Articles
- EP ID EP138712
- DOI -
- Views 146
- Downloads 0
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ETHINYL ESTRADIOL AND LEVONORGESTREL
ABSTRACT A simple, accurate, precise, sensitive, specific and reliable stability indicating RP-HPLC method was developed for simultaneous estimation of Ethinyl estradiol (EE) and Levonorgestrel (LEV) in Pharmaceuti...
IR INTERPRETATION, DERIVATIVE UV SPECTRA AND VALIDATED UV SPECTROSCOPY METHOD FOR THE ESTIMATION OF HALOPERIDOL IN TABLET DOSAGE FORM
ABSTRACT A simple, accurate and rapid UV method was validated with derivative spectra for the estimation of haloperidol in tablets. The drug is soluble in 0.1 N methanolic sulphuric acid and all the solutions wer...
ANALYTICAL TECHNIQUES FOR THE ESTIMATION OF FENOFIBRATE IN BULK AND PHARMACEUTICAL DOSAGE FORMS: A REVIEW
ABSTRACT Fenofibrate which is chemically propan-2-yl 2-{4-[(4-chlorophenyl) carbonyl] phenoxy}-methyl propanoate. It is mainly used to reduce cholesterol levels in patients at risk of cardiovascular disease. Like...
RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND TELMISARTAN IN TABLET DOSAGE FORM
ABSTRACT A simple, sensitive and rapid reverse phase HPLC method was developed for the simultaneous estimation of Amlodipine besylate and Telmisartan. A Phenominex-luna C18 column (250x4.6 mm i.d 5ยต) was used wit...
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS ESTIMATION OF RUPATADINE FUMARATE AND MONTELUKAST SODIUM BY RP-HPLC
ABSTRACT A sensitive, selective and precise high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Rupatidine fumarate and Montelukast sodium in tabl...