An approach for enhancing the production of human-like collagen II by enlarging the metabolic flux at pyruvate node
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2014, Vol 27, Issue 6
Abstract
As a key metabolite of glycolysis, Pyruvate affects cell growth and metabolism directly. In order to enhance the production of human-like collagen (HLC) II, pyruvate was added into fermentation broth, and response surface methodology (RSM) to optimize the addition time and dosage of pyruvate and the concentration of phosphate buffer. And the results of PB-design showed that only two variables ─ the dosage of pyruvate and the concentration of buffer ─ had significant effects on the production of human-like collagen. Quadratic polynomial models were established after analyzing the results of full factorial central composite design. The optimal dosage of pyruvate and the concentration of buffer were 8.23 mmol•L-1 and 0.17 mol•L-1 respectively. Under the optimal cultivation condition, Cell OD600 was increased 28.72%; the maximal production of human-like collagen could be up to 0.29 g•L-1 which was 11.54% higher than that of the control group 0.26 g•L-1 (the highest production of the former study).
Authors and Affiliations
MA Xiaoxuan , ZHU Chenhui , Shang Zifang , ZHANG Gaoping , FAN Daidi
Keywords
Related Articles
- EP ID EP142434
- DOI -
- Views 79
- Downloads 0
Synthesis, characterization and bioevaluation of technetium-99m labeled N-(2-Hydroxybenzyl)-2-amino-2-deoxy-D-glucose as a tumor imaging agent
N-(2-Hydroxybenzyl)-2-amino-2-deoxy-D-glucose (NHADG) was synthesized by conjugation of salicylaldehyde to glucosamine. The obtained compound was well characterized via different analytical techniques. Labeling of the sy...
A tentative component analysis of Norjizak: A new abused drug in Iran
Norjizak is a new drug abused in the past few years in Iran with symptoms and complications distinct from other common forms of drug and characterized by higher rate of mortality. The present study aims to analyze...
Antioxidant evaluation for Urtica urens, Rumex cyprius and Borago officinalis edible wild plants in Palestine
Natural plants products are one of the famous and commonly utilized remedies used in fighting diseases. This study was conducted to evaluate antioxidant activity of plants commonly used in Palestine (Urtica urens,...
Anticonvulsant and neuroprotective effects of the Acacia tortilis growing in KSA
In different region of Saudi Arabia Acacia tortilis (Fabaceae) is present but still the medicinal properties of Acacia tortilis have not been studied. However, in Zimbabwe different species of Acacia are already us...
Determination of pKa values of new phenacyl-piperidine derivatives by potentiometric titration method in aqueous medium at room temperature (25±0.5oC)
Dissociation constant (pKa) of ten novel phenacyl derivatives of piperidine were determined by potentiometric titration method in aqueous medium at room temperature (25 ±0.5oC). The sample solutions were prepared i...