Abstract

 ABSTRACT Flowcytometry is a powerful technology mainly used for investigations in cell biology and isolation of individual cells.It is a laser based biophysical analytical technique used mainly for cell sorting, cell counting, biomarker detection and protein engineering. It is one of the high powerful, sensitive and specific for simultaneous estimation of multiparameters of individual cells in heterogeneous suspension. Flowcytometry can analyze thousands of particles per second. In flowcytometry, analysis is done based on physical interaction of particles with laser light. It is used both for single cell analysis and multi cell analysis. Now a day’s flowcytometry has become an essential tool in the clinical field for diagnosis of different types of diseases. Because of this analytical capability, flowcytometry become an integral part in medical research centers .At the present time flowcytometry is available as a new and specialized flowcytometer called High Throughput Pharmacological System(HTPS).It is mainly used for high throughput analysis of 9-10 samples/minute. The flowcytometry is applicable to few branches of sciences both alone and in combination with other methods. It gives new and valuable results in clinical field that could not be obtained by any other analytical methods. Keywords: Cell sorting, Laser based technique, High Throughput analysis, Single cell analysis.

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