AN OVERVIEW ON HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS
Journal Title: Int J of Pharm Res & Analy - Year 2016, Vol 6, Issue 1
Abstract
ABSTRACT High performance Capillary electrophoresis (HPCE) is a separation technique in which the analytes are separated on the basis of differences in their charge-to-size ratios. This makes HPCE especially suitable for the analysis of molecules with abroad range of sizes, charge and hydrophobicity, as proteins and peptides. Another attractive feature of HPCE is its ability to handle minute sample amounts, with nano-litre injection volumes, which makes it ideal for applications when the sample volumes are limited, as often is the case in analysis of body fluids, single cells and other small volume analysis of biofluids. Electrophoresisisperformed innarrow-bore (25- to 75-J.m id), fused silica capillaries. High voltages (10 to 30 kV) and high electricfields (100 to 500 V/cm) are applied across the capillary. In addition, the small injection volume has often been considered as a drawback of the HPCE technique in analysis of complex biological samples, as well as the nature of its injection has represented a reproducibility problem in quantitative chemical analysis. Keywords: High performance, Capillary electrophoresis, Hydrophobicity, Injection, Electric field.
Authors and Affiliations
Sudheer Kumar N*, Shilpa KT, Ajitha A, Uma Maheshwara Rao V
Keywords
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