Analysis of genetics and chemical contents relation compared to commonly used Cissus quadrangularis L. and barcode markers of some Thailand Cissus species
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2016, Vol 29, Issue 1
Abstract
Several Cissus species are commonly used as traditional and modified medicines, and their chemical constituents are major point for precise usage. However, C. quadrangularis is the only species for which the usages and the chemical composition have been reported. These data should be investigated for other species in the genus. Eight species namely C. assamica, C. carnosa, C. elongata, C. hastata, C. javana, C. pteroclada, C. quadrangular is and C. repens were evaluated for genetic relationships and chemical composition. Constructed dendrogram shows high-powered efficiency of inter-simple sequence repeat (ISSR) data used which can clearly identify different and identical species. Genetic similarity (S) value of the identical species is 0.86, whereas for different species the value can vary from 0.53 to 0.75. Four highly related species (S=0.64-0.72), C. assamica, C. carnosa, C. hastata and C. repens were selected to undergo chemical study by gas chromatography-mass spectrometry (GC-MS) on the methanol crude extract. Only one compound, β-sitosterol, found in the four species is identical to the compound reported from C. quadrangular is, where there were five identical chemicals found in the selected species. Species-specific barcode with rbcL region was constructed. Nucleotide variation was evaluated indicating genetic distance value of 0.025 to 0.072.
Authors and Affiliations
Runglawan Sudmoon , Arunrat Chaveerach , Tawatchai Tanee
Keywords
Related Articles
- EP ID EP122983
- DOI -
- Views 84
- Downloads 0
Assessment of anti-inflammatory, anti-ulcer and neuro-pharmacological activities of Cyperus rotundus Linn.
This article reports the assessment of anti-inflammatory, antiulcer and neuropharmacological activities of crude extract of Cyperus rotundus. The plant exhibited significant property to act as an anti inflammatory...
Quality evaluation and in vitro interaction studies between levofloxacin 250mg and diclofenac sodium 50mg tablets
Fluoroquinolones are broad-spectrum antibiotics, work against Gram-positive and Gram-negative bacteria and are a clinically proven option for many resistant infections. Among fluoroquinolones Levofloxacin works bes...
Additional traditional Chinese medicine on gastrointestinal dysfunction in patients with sepsis: A systematic review and meta-analysis
To evaluate the clinical effect and safety of western medicine plus Traditional Chinese medicine for sepsis with gastrointestinal dysfunction. We searched CNKI (January 1979 to June 2014), VIP (January 1989 to June...
Cytotoxic flavonoids from the young twigs and leaves of Caesalpinia bonduc (Linn) Roxb
The extraction, fractionation and recognition of flavonoids from the ethanolic extract of young twigs and leaves of C. bonduc were carried out. In addition, cytotoxic study of the flavonoids on two cancer cell line...
Pioglitazone, a ppar-γ lıgand inhibited the nicotinamide-streptozotocin induced sperm abnormalities in type-2 dıabetic wistar rats
Oxidative stress due to chronic hyperglycemia is known to cause infertility in diabetic patients. Pioglitazone (PIO), a PPAR-γ ligand is known to possess the antioxidant property however, its role on the oxidative stress...