Antioxidant activity and fructan content in root extracts from elecampane (Inula helenium L.)

Journal Title: Journal of BioScience and Biotechnology - Year 2015, Vol 4, Issue 1

Abstract

 The purpose of the present study was to investigate the antioxidant activity and fructans content in ethanol and water extracts of roots of elecampane (Inula helenium L.), a Bulgarian medicinal plant. The extraction procedure included 95% (v/v) ethanol extraction and subsequent water treatment. The antioxidant activity was evaluated by several reliable methods such as DPPH-, ABTS-, FRAP- and CUPRAC-assays, as well as the total phenolic content. In addition, the total fructans and sugar content were determinated by spectrophotomeric, TLC and HPLC-RID methods. The level of fructans in ethanol extracts was 14.1 g / 100 g dry weight, as nystose and 1-kestose were only 0.3 g / 100 g dry weight, and 0.5 g / 100g dry weight, respectively. The absence of fructooligosacharides and sugars in water extracts after the ethanol pretreatment was established. Inulin content was evaluated to be 32 g / 100 g dry weight. The metabolites profile of roots revealed their potential application as radical scavengers due to the presence of polyphenols. Therefore, the root extracts of elecampane could be assumed as a rich source of biologically active substance, in particular dietary fiber with potential prebiotic effect, due to the presence of polysaccharide inulin and fructooligosacharides.

Authors and Affiliations

NADEZHDA PETKOVA, RADKA VRANCHEVA, DASHA MIHAYLOVA, IVAN IVANOV, ATANAS PAVLOV, PANTELEY DENEV

Keywords

Related Articles

 In vitro fermentation response to alkaline treated sorghum grain

 Effects of three alkaline treatments: NaOH, NaHCO3 and wood ash on the crude protein (CP), condensed tannin (CT), neutral detergent fiber (NDF), in vitro gas production kinetics and dry matter (DM) digestibility of...

Study of the transgalactosylation activity of β-galactosidase from a new strain Kluyveromyces lactis 3

Beta-galactosidase (EC.3.2.1.23) is an important enzyme industrially used for the hydrolysis of lactose from milk and milk whey for several applications. Lately, the importance of this enzyme was enhanced by its galactos...

Differential glycation of arginine and lysine by glucose and inhibition by acesulfame potassium

Glycation is a non-enzymatic process between the reactive carbonyl group of sugars and free amino groups of proteins especially arginine and lysine residues. This process leads to the formation of a group of compounds ca...

Allelopatic effects of some medicinal plant essential oils on plant seeds germination

The effect of essential oils from some medicinal plants on seed germination was studied with the aim of assessing their potential use as bioherbicides. The experiment was conducted as factorial based on completely random...

Download PDF file
  • EP ID EP137525
  • DOI -
  • Views 99
  • Downloads 0

How To Cite

NADEZHDA PETKOVA, RADKA VRANCHEVA, DASHA MIHAYLOVA, IVAN IVANOV, ATANAS PAVLOV, PANTELEY DENEV (2015).  Antioxidant activity and fructan content in root extracts from elecampane (Inula helenium L.). Journal of BioScience and Biotechnology, 4(1), 101-107. https://europub.co.uk/articles/-A-137525