Biochemical analysis of Casein polymorphism in Tunisian local Goat
Journal Title: Scientia Agriculturae - Year 2015, Vol 10, Issue 1
Abstract
Genetic diversity among local goat population from six localities (Elhamma, Hazoua, Degueche, Nefta, Tozeur, and Chabbat) in the south of Tunisia were estimated by biochemical analysis. Five proteins were identified by electrophoresis: alpha-s1 casein (CSNS1), beta-casein (CSN2), beta-Lacto-globulin (β-LG), alpha-lactalbumin(α-Lac) and serum albumin (SA). The inventory of the polymorphism was performed on the population of local goat showed that all the loci were polymorphs with an average of 2.4 alleles by locus. The results show that there are three allelic variants for the caseins CSNS1 and CSN2 with a predomination of the CSNS1-C for the alpha-S1 casein and the predomination of the CSN2-A variant for the beta-casein. Moreover, the observed heterozygosity values ranged from 0.45 in region of Elhamma to 0.52 in region of Hazoua. The mean estimates of FST was 0.0285 (p<0.05) and indicated that most 97.15% of total genetic variation originates from differences among individuals. Similarly, the study of the genetic distance of Nei (1972) reveals that a low heterogeneity between the different subpopulation (locality).This explains that the coefficient of similarity is approximately 1. Characterization of casein biochemical polymorphism can be a useful way to know genetic diversity status of local goat in Tunisia.
Authors and Affiliations
H Ben Zaabza, B Jemmali, M Nefti, K Zahrane
Keywords
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- EP ID EP127306
- DOI 10.15192/PSCP.SA.2015.10.1.3843
- Views 91
- Downloads 0
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