Cation-Chloride Co-Transporters mRNA Expression In Mice CA1 Region After Lithium-Pilocarpine Induced Status Epilepticus
Journal Title: Journal of Neurological Sciences-Turkish - Year 2012, Vol 29, Issue 2
Abstract
Objective: Intracellular chloride concentration of a given cell within the central nervous system is determined by a delicate balance between the Cl- extrusion system and the Cl- accumulation system. In this study, we aim to investigate the expression profiles of cation-chloride cotransporters (CCCs) mRNA in the mice model of lithium-pilocarpine induced status epilepticus (PISE) and the possible relationships between CCCs and epileptogenesis. Methods: Male adult Balb/c mice were randomly divided into two groups: the PISE group and the control. We used the mice of PISE group to establish the animal model of PISE and the PISE group could be subdivided into three time points: 1 d, 14 d and 45 d. Real-time fluorescence quantitative PCR (polymerase chain reaction) was used to explore the expression of CCCs family members mRNA in CA1 region. Results: These CCCs members all showed distinctive expression patterns. The expression levels of NKCC1 (sodium potassium cation-chloride cotransporter 1), KCC1 (potassium cation-chloride cotransporter 1), KCC3 (potassium cation-chloride cotransporter 3) and CIP1 (CCC-interacting protein type 1)mRNA were up-regulated while those of KCC2 (potassium cation-chloride cotransporter 2) and CCC9 (cation-chloride cotransporter) mRNA were down-regulated after PISE. The expression of KCC4 (potassium cation-chloride cotransporter 4) remained unchanged. Conclusions: These data lead us to conclude that CCCs expression as a whole is under very strict transcriptional control in this model. Deregulation of their expression might break the balance of intracellular and extracellular chloride concentration which contributed to the mechanism of hyperexcitability that led to seizures. This may be due to some form of genetic program activated in response to epileptic seizure in the brain and may highlight which biological pathways are modulated.
Authors and Affiliations
Li XIUBIN, Zhou LIEMIN
Keywords
Related Articles
- EP ID EP146146
- DOI -
- Views 70
- Downloads 0
Optik Nörit Şeklinde Ortaya Çıkan Klinik İzole Sendromlu Bir Hastada Steroid Tedavisi Sonrası Psikotik Atak
Giriş: Kortikosteroidler yaygın olarak Multipl Skleroz'da (MS) atak tedavisinde ve Klinik İzole Sendromlarda kullanılmaktadır ancak nadiren depresyon, mani, psikoz ve deliryum gibi psikiyatrik tablolara neden olurlar. St...
Bir Katamenial Epilepsi Olgusu: Fenobarbital Tedavisine İyi Yanıt
Katamenial epilepsi (KE) epileptik nöbetlerin menstrüel siklus döneminde veya hemen öncesinde yaşandığı epilepsi tipidir. Tanısı epileptik nöbetler için günlük tutulması ile nöbetlerin menstrüel sikluslarla ilişkisinin g...
Subakut Kombine Dejenerasyon: Spinal MRI ve Klinik Özellikleri İle Bir Olgu
Myelopati bulguları ile başvuran 66 yaşında bayan hastada yapılan ile vitamin B12 yetmezliği olduğu ortaya komuştur. Spinal MRI da T2 ağırlıklı kesitlerde servikal ve torakal kord dorsalinde hiperintens lezyonlar saptanm...
Gerstmann Syndrome; A Frequent Pathology Presenting With A Rare Symptomatology
We report a case of glioblastoma multiforme (GBM) of the left parietal lobe presenting with Gerstmann Syndrome (GS). A 54-year-old woman presented dyscalculia. The computed tomography depicted a cystic mass in the left p...
Porphyria with predominantly involvement of bilateral facial nerves
Porphyria is an uncommon disease caused by the deficient activity of a heme biosynthetic enzyme, which presents clinically with neurological and psychiatric symptoms. To our knowledge, this is the first report describing...