Cation-Chloride Co-Transporters mRNA Expression In Mice CA1 Region After Lithium-Pilocarpine Induced Status Epilepticus
Journal Title: Journal of Neurological Sciences-Turkish - Year 2012, Vol 29, Issue 2
Abstract
Objective: Intracellular chloride concentration of a given cell within the central nervous system is determined by a delicate balance between the Cl- extrusion system and the Cl- accumulation system. In this study, we aim to investigate the expression profiles of cation-chloride cotransporters (CCCs) mRNA in the mice model of lithium-pilocarpine induced status epilepticus (PISE) and the possible relationships between CCCs and epileptogenesis. Methods: Male adult Balb/c mice were randomly divided into two groups: the PISE group and the control. We used the mice of PISE group to establish the animal model of PISE and the PISE group could be subdivided into three time points: 1 d, 14 d and 45 d. Real-time fluorescence quantitative PCR (polymerase chain reaction) was used to explore the expression of CCCs family members mRNA in CA1 region. Results: These CCCs members all showed distinctive expression patterns. The expression levels of NKCC1 (sodium potassium cation-chloride cotransporter 1), KCC1 (potassium cation-chloride cotransporter 1), KCC3 (potassium cation-chloride cotransporter 3) and CIP1 (CCC-interacting protein type 1)mRNA were up-regulated while those of KCC2 (potassium cation-chloride cotransporter 2) and CCC9 (cation-chloride cotransporter) mRNA were down-regulated after PISE. The expression of KCC4 (potassium cation-chloride cotransporter 4) remained unchanged. Conclusions: These data lead us to conclude that CCCs expression as a whole is under very strict transcriptional control in this model. Deregulation of their expression might break the balance of intracellular and extracellular chloride concentration which contributed to the mechanism of hyperexcitability that led to seizures. This may be due to some form of genetic program activated in response to epileptic seizure in the brain and may highlight which biological pathways are modulated.
Authors and Affiliations
Li XIUBIN, Zhou LIEMIN
Keywords
Related Articles
- EP ID EP146146
- DOI -
- Views 84
- Downloads 0
Co-occurence of Auto-Immune Thyroid Disease and Acromegaly
Objective: The aim of the paper is to discuss the co-occurence of thyroid disease especially the autoimmune ones and acromegaly in conjunct of two acromegalic patient who developed autoimmune thyroid disease. Material...
Pretest Results of The Revised Standardized Mini Mental Examination Test In Community Dwelling Elderly
Aim. To examine the Turkish version of Standardized Mini Mental State Examination (SMMSE) that some items were revised in the community dwelling elderly population as a pretest. Methods. The revised SMMSE that some it...
İntramusküler Thiocolchicoside Enjeksiyonu Sonrası Gelişen Ensefalopati Tablosu
Deliryum, mental durumda günler veya saatler süren akut değişiklikler ile karakterizedir. Deliryumlu hastaların yaklaşık %30'unda neden ilaç toksisitesidir. Tıbbi öykü dikkatlice gözden geçirilmeli, semptomların ba...
Multipl Skleroz'da Doğal Seyir ve Klinik Prognostik Özellikler
Amaç: Multipl skleroz(MS), farklı klinik seyir ve prognoz özellikleri gösterebilen heterojen hastalık grupları içermektedir. Hastalığın erken dönemindeki verilere dayanarak prognozu önceden belirleyebilmek MS çalışmaları...
Heart Rate and QT interval Variability in Multiple Sclerosis: Evidence for Decreased Sympathetic Activity
Decreased beat-to-beat heart rate variability (HR) and increased beat-to-beat QT interval variability are associated with significant cardiovascular mortality. The aim of this study was to compare beat-to-beat HR and QT...