rAAV2-mediated Gene Expression In Primary Cortical Neural Cells Following Inhibition Of DNA Synthesis and/or EGFR Tyrosine Kinase
Journal Title: Journal of Neurological Sciences-Turkish - Year 2005, Vol 22, Issue 2
Abstract
Aim: In an effort to enhance rAAV2 transduction efficiency in primary cortical neural cells, we examined the combined effects of chemicals known to increase rAAV2 transduction efficiency in many other cell types. Results: Unexpectedly, pretreatment of neural cells with genotoxic agents (hydroxyurea or aphidicolin) and/or the EGFR tyrosine kinase inhibitor, tyrphostin-1, did not improve transduction efficiency. SK-Hep1 cells expressing dramatic increase in rAAV2 transduction following chemical combination, experienced both significant release from G1/G0 phase and phosphorylation of p38, whereas no in neural cells. Conclusion: Our results suggest that chemical pretreatment with hydroxyurea, aphidicolin and/or tyrphostin-1 is unable to increase rAAV2 transduction efficiency in neural cells, indicating that other strategies should be investigated
Authors and Affiliations
Heuiran LEE, Young NAM, Sung KIM, Dong-Hou KIM, Yunhee KWON, Yoo KIM
Keywords
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