CHARACTERIZATION OF L-ASPARAGINASE PRODUCING ENDOPHYTIC FUNGI ISOLATED FROM RIPENED FRUIT OF CAPSICUM FRUTESCENCE
Journal Title: International Journal of Pharmaceutical Development & Technology - Year 2014, Vol 4, Issue 1
Abstract
The main objective is to evaluate L-asparaginase production of endophytic fungi isolated from ripened fruit of Capsicum frutescence var US 341. In the present work endophytic fungal strains were screened for production of extra-cellular L-asparaginase, an anti-neoplastic agent used in the chemotherapy of lymphoblastic leukemia. L-Asparaginase activity of endophytic fungi was detected using modified Czapex dok’s medium containing L-asparagine and Phenol red as indicator. In the present study out of 16 fungal isolates, 6 fungal isolates were identified as L-asparaginase positive of which two fungal isolates RFEF-1 and RFEF-2 are identified as Aspergillus sp on their morphological characterization of mycelia, conidiospores and conidia under microscopic observation. The effect of growth parameters on biomass and production of L-asparaginase of these isolates have been characterized. Both isolates showed maximum biomass RFEF-1(2.42gms), RFEF-2(3.03gms) and L-asparaginase production RFEF-1(39.7 IU), RFEF-2(37.86 IU) at 35oC and pH -7.0.
Authors and Affiliations
Amrutha V. Audipudi , G. Naga Ratna Supriya , R. Pallavi , P. Ganga Mani
Keywords
Related Articles
- EP ID EP131992
- DOI -
- Views 93
- Downloads 0
DEVELOPMENT AND CHARACTERIZATION OF LOVASTATIN LOADED MICROSPHERES
In this study, the use of biodegradable polymers for microencapsulation of Lovastatin using solvent evaporation technique was investigated. Ethyl Cellulose and Eudragit L100 was selected as a retardant polymer be...
IN-VITRO ANTI INFLAMMATORY ACTIVITY OF ULTRA SONIC BATH ASSISTED, METHANOL EXTRACT OF LEPIDIUM SATIVUM LINN. SEEDS
Plants can provide biologically active molecules and lead structures for the development of modified derivatives with enhanced activity and reduced toxicity. There are certain problems associated with use of anim...
BIODEGRADABLE POLYMER MICRONEEDLES FOR CONTROLLED-RELEASE DRUG DELIVERY
Biopharmaceuticals, which include proteins, DNA, vaccines and other biologically related molecules, make up one of the fastest growing segments of the overall pharmaceutical market. However, there are significant d...
ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY OF LEAF EXTRACT OF MORINDA CITRIFOLIA IN EXPERIMENTAL MODELS
Morinda citrifolia is a medium sized evergreen tree belongs to Rubiaceae family. The petroleum ether extract ofMorinda citrifolia (PMC) leaves was investigated for the evaluation of anti- inflammatory and analgesic...
STUDIES ON THE CHEMICAL CHARACTERISTICS OF GROUNDNUT OIL
ABSTRACT Vegetable oil had made an important contribution to the diet in many countries. Its constitute one of the essential components of balanced diet as good source of energy. The chemical and physical propert...