Chromatographic methods for simultaneous determination of diiodohydroxyquinoline and Metronidazole in their binary mixture
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2013, Vol 26, Issue 5
Abstract
Two chromatographic methods were developed for analysis ofdiiodohydroxyquinoline (DIHQ) and metronidazole (MTN). In the first method, diiodohydroxyquinoline and metronidazole were separated on TLC silica gel 60F254 plate using chloroform: acetone: glacial acetic acid (7.5: 2.5: 0.1, by volume) as mobile phase. The obtained bands were then scanned at 254 nm. The second method is a RP-HPLC method in which diiodohydroxyquinoline and metronidazole were separated on a reversed-phase C18 column using water : methanol (60 :40, V/V, PH=3.6 )as mobile phase at a flow rate of 0.7 mL.min-1 and UV detection at 220 nm. The mentioned methods were successfully used for determination of diiodohydroxyquinoline and metronidazole in pure form and in their pharmaceutical formulation.
Authors and Affiliations
Nouruddin Ali, Mohammed Gamal
Keywords
Related Articles
- EP ID EP125744
- DOI -
- Views 99
- Downloads 0
Formulation and evaluation of a single vial lyophilized preparation of technetium-99m labeled ethylene dicysteine as a renal scintigraphic agent
99mTc labeled N-N-ethylene-L, L-dicysteine (EC) was introduced in form of multiple-step kit as an alternate renal imaging radiopharmaceutical for commonly used 99mTc-MAG3. We developed a single component lyophilize...
Prevalence and susceptibility patterns of bacteria causing respiratory tract infections in North Waziristan, Pakistan
Respiratory tract infections (RTIs) are the most common infectious diseases in humans and are the major cause of mortality and morbidity in Pakistan. These infections are the leading causes of consultations in prim...
Abnormal expressions of inflammatory-related mediators and inhibition of fat metabolism in mice infected with influenza a virus
The pathophysiological role of influenza infection is poorly understood. In this study, one non-neurovirulent virus (IAV/Aichi/2/68/H3N2) strain was used to infect intra-nasally mice at different age to investigate...
Acoustical behavior of some amino acids in aqueous disodium citrate solutions over temperature range (298.15-313.15) K
Sound velocity, U, and density, ρ, of some amino acids viz. glycine, methionine, phenylalanine and tryptophan were determined in 0.1, 0.2 and 0.3 mol kg−1aqueous disodium citrate solutions as a function of concentr...
Analysis of genetics and chemical contents relation compared to commonly used Cissus quadrangularis L. and barcode markers of some Thailand Cissus species
Several Cissus species are commonly used as traditional and modified medicines, and their chemical constituents are major point for precise usage. However, C. quadrangularis is the only species for which the usages...